The Secretion of α -Ecdysone by the Prothoracic Glands of Manduca sexta in Vitro

Prothoracic glands of tobacco hornworm larvae cultured in vitro secrete into the culture medium a substance which was active in ecdysone bioassays and determined to be ecdysone-like by radioimmunoassay. The prothoracic glands appear to be the sole source of this substance. The material was identifie...

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Published inProceedings of the National Academy of Sciences - PNAS Vol. 71; no. 3; pp. 793 - 796
Main Authors King, D. S., Bollenbacher, W. E., Borst, D. W., Vedeckis, W. V., O'Connor, J. D., Ittycheriah, P. I., Gilbert, L. I.
Format Journal Article
LanguageEnglish
Published United States National Academy of Sciences of the United States of America 01.03.1974
National Acad Sciences
Subjects
Animal glands
Bioassay
Biological Sciences: Zoology
Chromatography
Hormones
Insect hormones
Insect larvae
Molting
Prothoracic glands
Radioimmunoassay
Tissue culture techniques
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Summary:Prothoracic glands of tobacco hornworm larvae cultured in vitro secrete into the culture medium a substance which was active in ecdysone bioassays and determined to be ecdysone-like by radioimmunoassay. The prothoracic glands appear to be the sole source of this substance. The material was identified as α -ecdysone by thin-layer, gas-liquid, and high-resolution liquid chromatography and mass spectrometry. No other active moulting principles were present in the medium. It appears that the prothoracic gland secretes a prohormone, α -ecdysone, which is subsequently converted into the active moulting hormone, β -ecdysone, in other insect tissues.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.71.3.793