Biopolymer nanoparticles designed for polyunsaturated fatty acid vehiculization: Protein–polysaccharide ratio study

• Published in: Food chemistry Vol. 188; pp. 543 - 550
• Main Authors: Perez, Adrián A., Sponton, Osvaldo E., Andermatten, Romina B., Rubiolo, Amelia C., Santiago, Liliana G.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.12.2015
• Subjects: Binding properties; Biopolymer nanoparticles; Biopolymers - chemistry; Fatty Acids, Unsaturated - chemistry; High methoxyl pectin; Lactoglobulins - chemistry; Linoleic acid; Nanoparticles - chemistry; Pectins - chemistry; Polysaccharides - chemistry; Vehiculization; β-Lactoglobulin; Vehiculization; High methoxyl pectin; Binding properties; β-Lactoglobulin; Linoleic acid; Biopolymer nanoparticles
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2015.05.043

•Protein–polysaccharide nanoparticles (BNPs) for PUFA vehiculization were designed.•Linoleic acid (LA) was used as model PUFA.•Stable BNPs were obtained at 1:1 and 2:1 protein–polysaccharide ratio.•Coacervation was observed at 4:1 and 6:1 protein–polysaccharide ratio.•At 2:1 protein–polysaccharide ratio a great LA protection in BNPs was observed. Information about the design of biopolymer nanoparticles (BNPs) for polyunsaturated fatty acid (PUFA) vehiculization is provided. Linoleic acid (LA) was used as a model PUFA. The binding ability of LA to β-lactoglobulin (BLG) was applied for obtaining BLG–LA complexes. BLG–LA complex formation was monitored by fluorimetry and it was observed that a moderate heat treatment (60°C, 10min) enhanced BLG–LA complexation. Obtaining BNPs involved the electrostatic deposition of high methoxyl pectin (HMP) onto the BLG–LA complex surface. The phase behavior of biopolymer systems was discussed at different Prot:HMP ratio (RProt:HMP, wt.%) levels (1:1–6:1). Absorbance at 600nm, particle size, and ζ potential were analyzed at pH 4.0. At 1:1–2:1 RProt:HMP, BNPs showed appreciable turbidity, a nanometric diameter (337–364nm), and a negative ζ potential. Finally, intrinsic and extrinsic fluorimetry was used for examining the HMP protective role at the LA binding site. At 2:1 RProt:HMP, HMP cover could promote significant LA protection in BNPs.