Rapid detection of herpes simplex virus DNA in cerebrospinal fluid : Comparison between loop-mediated isothermal amplification and real-time PCR

• Published in: Medical microbiology and immunology Vol. 194; no. 4; pp. 181 - 185
• Main Authors: KIMURA, Hiroshi, IHIRA, Masaru, ENOMOTO, Yoshihiro, KAWADA, Jun-Ichi, ITO, Yoshinori, MORISHIMA, Tsuneo, YOSHIKAWA, Tetsushi, ASANO, Yoshizo
• Format: Journal Article
• Language: English
• Published: Berlin Springer 01.08.2005; Springer Nature B.V
• Subjects: Acids; Adolescent; Adult; Aged; Biological and medical sciences; Central Nervous System Viral Diseases - cerebrospinal fluid; Central Nervous System Viral Diseases - diagnosis; Child; Deoxyribonucleic acid; DNA; DNA, Viral - cerebrospinal fluid; Female; Fundamental and applied biological sciences. Psychology; Herpes Simplex - cerebrospinal fluid; Herpes Simplex - diagnosis; Herpes simplex virus; Herpes viruses; Humans; Infant; Infant, Newborn; Male; Microbiology; Middle Aged; Miscellaneous; Nucleic Acid Amplification Techniques; Polymerase Chain Reaction; Predictive Value of Tests; Research methodology; Sensitivity and Specificity; Simplexvirus - genetics; Simplexvirus - isolation & purification; Virology; Encephalitis; Nervous system diseases; Microbiology; Herpesviridae; Alphaherpesvirinae; HSV; Cerebrospinal fluid; Real time; Cerebral disorder; Virus; Amplification; Polymerase chain reaction; Immunology; Real-time PCR; Central nervous system disease; CSF; Herpesvirus hominis; LAMP; Detection
• ISSN: 0300-8584; 1432-1831
• DOI: 10.1007/s00430-005-0242-9

Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification method that amplifies DNA with high specificity, efficiency, and speed under isothermal conditions. To evaluate the usefulness of LAMP for diagnosing central nervous system infection with herpes simplex virus (HSV), we compared the LAMP method with real-time PCR, using samples that were previously tested by nested PCR. We examined 69 cerebrospinal fluid (CSF) samples from patients suspected of having HSV infection of the central nervous system. The results of the real-time PCR analysis and nested PCR assay were in complete accord. When nested PCR was regarded as standard, the sensitivity of LAMP was 81%, the specificity was 100%, the positive predictive value was 100%, and the negative predictive value was 90%. Although further improvement is necessary for the wide spread use, the LAMP method might be applicable to diagnosis of HSV infection of the central nervous system.