Human Immune Responses to a Novel Norwalk Virus Vaccine Delivered in Transgenic Potatoes
A new approach for delivering vaccine antigens is the use of inexpensive, plentiful, plantbased oral vaccines. Norwalk virus capsid protein (NVCP), assembled into virus-like particles, was used as a test antigen, to determine whether immune responses could be generated in volunteers who ingested tra...
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Published in | The Journal of infectious diseases Vol. 182; no. 1; pp. 302 - 305 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Chicago, IL
The University of Chicago Press
01.07.2000
University of Chicago Press Oxford University Press |
Subjects | |
Online Access | Get full text |
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Summary: | A new approach for delivering vaccine antigens is the use of inexpensive, plentiful, plantbased oral vaccines. Norwalk virus capsid protein (NVCP), assembled into virus-like particles, was used as a test antigen, to determine whether immune responses could be generated in volunteers who ingested transgenic potatoes. Twenty-four healthy adult volunteers received 2 or 3 doses of transgenic potato (n = 20) or 3 doses of wild-type potato (n = 4). Each dose consisted of 150 g of raw, peeled, diced potato that contained 215–751 μg of NVCP. Nineteen (95%) of 20 volunteers who ingested transgenic potatoes developed significant increases in the numbers of specific IgA antibody-secreting cells. Four (20%) of 20 volunteers developed specific serum IgG, and 6 (30%) of 20 volunteers developed specific stool IgA. Overall, 19 of 20 volunteers developed an immune response of some kind, although the level of serum antibody increases was modest. |
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Bibliography: | istex:BE7F2229059DD8395A20468041E79EB9A23FDA5B ark:/67375/HXZ-9V1TL68K-D ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-News-3 |
ISSN: | 0022-1899 1537-6613 |
DOI: | 10.1086/315653 |