Fluorescence-, isotope- or biotin-labeling of the 5′-end of single-stranded DNA/RNA using T4 RNA ligase

A rapid 5′-labeling method of single-stranded DNA/RNA was developed, which is based on the utilization of an adenylated intermediate in the reaction of T4 RNA ligase. This method is commonly useful for fluorescence-, isotope- or biotin-labeling of the 5′-ends of both oligo- and polynucleotides.

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Bibliographic Details
Published inNucleic acids research Vol. 25; no. 18; pp. 3747 - 3748
Main Authors Kinoshita, Yasunori, Nishigaki, Koichi, Husimi, Yuzuru
Format Journal Article
LanguageEnglish
Published England Oxford University Press 15.09.1997
Subjects
Biotin
DNA - chemistry
Fluorescent Dyes
Isotope Labeling
Molecular Probe Techniques
Phage t4
RNA - chemistry
RNA Ligase (ATP)
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Summary:A rapid 5′-labeling method of single-stranded DNA/RNA was developed, which is based on the utilization of an adenylated intermediate in the reaction of T4 RNA ligase. This method is commonly useful for fluorescence-, isotope- or biotin-labeling of the 5′-ends of both oligo- and polynucleotides.
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ISSN:0305-1048
1362-4962
DOI:10.1093/nar/25.18.3747