Differentiating blood samples from scrapie infected and non-infected hamsters by detecting disease-associated prion proteins using Multimer Detection System

This communication describes the application of a modified sandwich enzyme-linked immunosorbent assay (ELISA), termed Multimer Detection System (MDS) for the detection of disease-associated multimeric forms of the prion protein (PrPd) in hamster blood. PrPd was detected in plasma of prion-affected h...

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Published inBiochemical and biophysical research communications Vol. 392; no. 4; pp. 505 - 509
Main Authors An, S.S.A., Lim, K.T., Oh, H.J., Lee, B.S., Zukic, E., Ju, Y.R., Yokoyama, T., Kim, S.Y., Welker, E.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 19.02.2010
Subjects
Aggregation
Animals
Antibody
Blood plasma
Cricetinae
Detection
Disease Models, Animal
ELISA
Enzyme-Linked Immunosorbent Assay
Monomer
Multimers
Prion
Protein Multimerization
PrPSc Proteins - blood
PrPSc Proteins - chemistry
Scrapie - blood
TSE
Aggregation
Antibody
TSE
Prion
Detection
Monomer
Multimers
Blood plasma
ELISA
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Summary:This communication describes the application of a modified sandwich enzyme-linked immunosorbent assay (ELISA), termed Multimer Detection System (MDS) for the detection of disease-associated multimeric forms of the prion protein (PrPd) in hamster blood. PrPd was detected in plasma of prion-affected hamsters while MDS revealed no PrPd in identically-treated plasma of healthy animals. This is the first report of a single ELISA- based immune detection of PrPd from blood samples.
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ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2010.01.053