A novel method to isolate cells with conditional gene expression using fluorescence activated cell sorting (FACS)

An inducible expression system using control elements of the tetracycline resistance operon has recently shown promise for conditional gene expression of any gene of interest. However, intensive screening of multiple independent clones is often required to find cell lines with optimal induction char...

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Bibliographic Details
Published inNucleic acids research Vol. 25; no. 23; p. 4858
Main Authors Klucher, Kevin M., Gerlach, Melissa J., Daley, George Q.
Format Journal Article
LanguageEnglish
Published England Oxford University Press 01.12.1997
Subjects
Animals
Cell Separation - methods
Flow Cytometry - methods
Fusion Proteins, bcr-abl - genetics
Gene Expression Regulation
Humans
Mice
Operon
Tetracycline Resistance - genetics
Tumor Cells, Cultured
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Summary:An inducible expression system using control elements of the tetracycline resistance operon has recently shown promise for conditional gene expression of any gene of interest. However, intensive screening of multiple independent clones is often required to find cell lines with optimal induction characteristics. By coupling expression of the gene of interest with a fluorescent marker, we have developed a novel fluorescence activated cell sorting (FACS) based strategy to isolate cells with desirable expression characteristics, thus alleviating the laborious isolation and analysis of multiple independent clones.
Bibliography:istex:C8AC38F2DDB716C72FA303E5B0EBE8F386E5FA62
ark:/67375/HXZ-QRHJN8VJ-3
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ISSN:0305-1048
1362-4962
1362-4962
DOI:10.1093/nar/25.23.4858