Identification of Chlorophyll Metabolism- and Photosynthesis-Related Genes Regulating Green Flower Color in Chrysanthemum by Integrative Transcriptome and Weighted Correlation Network Analyses

• Published in: Genes Vol. 12; no. 3; p. 449
• Main Authors: Fu, Hansen, Zeng, Tuo, Zhao, Yangyang, Luo, Tingting, Deng, Huijie, Meng, Chenwei, Luo, Jing, Wang, Caiyun
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI 21.03.2021; MDPI AG
• Subjects: chlorophyll metabolism; florist’s chrysanthemum; green ray floret; photosynthesis; segregating population; transcriptome; green ray floret; florist’s chrysanthemum; photosynthesis; transcriptome; weighted gene co-expression network analysis (WGCNA); chlorophyll metabolism; segregating population
• ISSN: 2073-4425; 2073-4425
• DOI: 10.3390/genes12030449

Green chrysanthemums are difficult to breed but have high commercial value. The molecular basis for the green petal color in chrysanthemum is not fully understood. This was investigated in the present study by RNA sequencing analysis of white and green ray florets collected at three stages of flower development from the F progeny of the cross between "Lüdingdang" with green-petaled flowers and with white-petaled flowers. The chlorophyll content was higher and chloroplast degradation was slower in green pools than in white pools at each developmental stage. Transcriptome analysis revealed that genes that were differentially expressed between the two pools were enriched in pathways related to chlorophyll metabolism and photosynthesis. We identified the transcription factor genes , , , and as regulators of the green flower color in chrysanthemum by differential expression analysis and weighted gene co-expression network analysis. These findings can guide future efforts to improve the color palette of chrysanthemum flowers through genetic engineering.