Genome-Enabled Estimates of Additive and Nonadditive Genetic Variances and Prediction of Apple Phenotypes Across Environments

• Published in: G3 : genes - genomes - genetics Vol. 5; no. 12; pp. 2711 - 2718
• Main Authors: Kumar, Satish, Molloy, Claire, Muñoz, Patricio, Daetwyler, Hans, Chagné, David, Volz, Richard
• Format: Journal Article
• Language: English
• Published: United States Genetics Society of America 01.12.2015
• Subjects: Algorithms; Breeding; Environment; Evolution, Molecular; Gene-Environment Interaction; Genetic Variation; Genetics, Population; Genome, Plant; Genomic Selection; Genomics; Inheritance Patterns; Malus - genetics; Models, Genetic; Pedigree; Phenotype; Polymorphism, Single Nucleotide; Reproducibility of Results; Selection, Genetic; GBLUP; genomic prediction; apple breeding; Malus × domestica; heritability
• ISSN: 2160-1836; 2160-1836
• DOI: 10.1534/g3.115.021105

The nonadditive genetic effects may have an important contribution to total genetic variation of phenotypes, so estimates of both the additive and nonadditive effects are desirable for breeding and selection purposes. Our main objectives were to: estimate additive, dominance and epistatic variances of apple (Malus × domestica Borkh.) phenotypes using relationship matrices constructed from genome-wide dense single nucleotide polymorphism (SNP) markers; and compare the accuracy of genomic predictions using genomic best linear unbiased prediction models with or without including nonadditive genetic effects. A set of 247 clonally replicated individuals was assessed for six fruit quality traits at two sites, and also genotyped using an Illumina 8K SNP array. Across several fruit quality traits, the additive, dominance, and epistatic effects contributed about 30%, 16%, and 19%, respectively, to the total phenotypic variance. Models ignoring nonadditive components yielded upwardly biased estimates of additive variance (heritability) for all traits in this study. The accuracy of genomic predicted genetic values (GEGV) varied from about 0.15 to 0.35 for various traits, and these were almost identical for models with or without including nonadditive effects. However, models including nonadditive genetic effects further reduced the bias of GEGV. Between-site genotypic correlations were high (>0.85) for all traits, and genotype-site interaction accounted for <10% of the phenotypic variability. The accuracy of prediction, when the validation set was present only at one site, was generally similar for both sites, and varied from about 0.50 to 0.85. The prediction accuracies were strongly influenced by trait heritability, and genetic relatedness between the training and validation families.