Human Intestinal Parasite Burden and Poor Sanitation in Rural Alabama

• Published in: The American journal of tropical medicine and hygiene Vol. 97; no. 5; pp. 1623 - 1628
• Main Authors: McKenna, Megan L, McAtee, Shannon, Bryan, Patricia E, Jeun, Rebecca, Ward, Tabitha, Kraus, Jacob, Bottazzi, Maria E, Hotez, Peter J, Flowers, Catherine C, Mejia, Rojelio
• Format: Journal Article
• Language: English
• Published: United States The American Society of Tropical Medicine and Hygiene 08.11.2017
• Subjects: Adolescent; Adult; Aged; Aged, 80 and over; Alabama; Animals; Child; Child, Preschool; Cryptosporidium - isolation & purification; Entamoeba histolytica - isolation & purification; Feces - parasitology; Female; Hookworm Infections - diagnosis; Hookworm Infections - epidemiology; Humans; Intestinal Diseases, Parasitic - diagnosis; Intestinal Diseases, Parasitic - epidemiology; Intestines - parasitology; Male; Middle Aged; Necator americanus - isolation & purification; Parasite Load; Prevalence; Rural Population; Sanitation; Soil - chemistry; Soil - parasitology; Strongyloides stercoralis - isolation & purification; Surveys and Questionnaires; Young Adult
• ISSN: 0002-9637; 1476-1645
• DOI: 10.4269/ajtmh.17-0396

Hookworm infection affects 430 million people worldwide, causing iron deficiency, impaired cognitive development, and stunting in children. Because of the environmental conditions needed for the hookworm life-cycle, this parasite is endemic to resource-limited countries. was endemic in the southern United States before improvement of sewage disposal systems and eradication programs. With continued poverty, poor sanitation, and an environment suitable for the hookworm life-cycle in some regions of the southern United States, a current prevalence study using modern molecular diagnostics is warranted. Lowndes County, Alabama, was chosen as the study site given previous high hookworm burdens, degree of poverty, and use of open-sewage systems. Participants were interviewed, and stool, serum, and soil samples were tested for nine intestinal parasites using a multiparallel quantitative real-time PCR (qPCR) and enzyme-linked immunosorbent assays. We found that, among 24 households, 42.4% reported exposure to raw sewage within their home, and from 55 stool samples, 19 (34.5%) tested positive for , four (7.3%) for , and one (1.8%) for . Stool tested positive for contained low levels of parasite DNA (geometric mean 0.0302 fg/μL). Soil studies detected one (2.9%) species, and serology assay detected one (5.2%) positive in this population. Individuals living in this high-risk environment within the United States continue to have stool samples positive for . Gastrointestinal parasites known to be endemic to developing countries are identifiable in American poverty regions, and areas with lower disease burden are more likely to be identified by using qPCR.