N-Sulfation of Heparan Sulfate with Microsomal Fraction of Endometrium of Rabbit Uterus

• Published in: The Tohoku Journal of Experimental Medicine Vol. 147; no. 3; pp. 295 - 300
• Main Authors: MUNAKATA, HIROSHI, ISEMURA, MAMORU, AIKAWA, JUNICHIRO, KOSAKAI, MASASHI, YOSIZAWA, ZENSAKU
• Format: Journal Article
• Language: English
• Published: Japan Tohoku University Medical Press 01.01.1985
• Subjects: Animals; endometrium; Endometrium - enzymology; Female; Glycosaminoglycans - biosynthesis; heparan sulfate; Heparitin Sulfate - analogs & derivatives; Heparitin Sulfate - biosynthesis; Heparitin Sulfate - metabolism; In Vitro Techniques; Microsomes - enzymology; Rabbits; Substrate Specificity; sulfotransferase; Sulfurtransferases - metabolism
• ISSN: 0040-8727; 1349-3329
• DOI: 10.1620/tjem.147.295

MUNAKATA, H., ISEMURA, M., AIKAWA, J., KOSAKAI, M. and YOSIZAWA, Z. N-Sulfation of Heparan Sulfate with Microsomal Fraction of Endometrium of Rabbit Uterus. Tohoku J. exp. Med., 1985, 147 (3), 295-300 - Heparan sulfate was prepared from the pronase digest of the porcine kidney and modified chemically to yield N-desulfated, N-, O-desulfated, and N-O-desulfated N-acetylated heparan sulfates. Heparan sulfate and its modifications thus obtained were used as substrates to measure the sulfotransferase activity in the microsomal fraction of the endometrium of the rabbit uterus. N-, O-Desulfated heparan sulfate was the best acceptor of sulfate from 3'-phosphoadenylyl [35S] sulfate and intact heparan sulfate was the poorest one. The products of enzymatic reaction were treated with nitrous acid and examined by gel-filtration and electrophoresis. The results indicated that sulfate was almost exclusively incorporated as N-sulfate into all the present substrates.