Detection of 3,6-dinitrobenzo[ a]pyrene in airborne particulates

• Published in: Mutation research Vol. 263; no. 1; pp. 27 - 32
• Main Authors: Sera, Nobuyuki, Kai, Mamiko, Horikawa, Kazumi, Fukuhara, Kiyoshi, Miyata, Naoki, Tokiwa, Hiroshi
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.05.1991; Elsevier
• Subjects: 3,6-Dinitrobenzo[ a]pyrene; Air Pollutants - toxicity; Airborne material; Benzo(a)pyrene - toxicity; Biological and medical sciences; Chemical mutagenesis; Chromatography, High Pressure Liquid; Gas Chromatography-Mass Spectrometry; Magnetic Resonance Spectroscopy; Medical sciences; Mutagenicity; Mutagens; Salmonella typhimurium - genetics; Toxicology; Airborne material; Mutagenicity; 3,6-Dinitrobenzo[ a]pyrene; South America; Ames test; Mutagenicity testing; Atmosphere; America; NMR spectrometry; Chile; Mutagen; Degradation product; Derived product; Mass spectrometry; Quantitative analysis
• ISSN: 0165-7992; 0027-5107
• DOI: 10.1016/0165-7992(91)90030-8

3,6-Dinitrobenzo[ a]pyrene, a new mutagen, was detected in airborne particulates collected in Santiago (Chile). The quantity of the compound in the airborne particulates was very small, accounting for 0.01 μg/g of total particulates (0.002 ng/m 3 of air) at the lowest concentration. It was found that 3,6-dinitrobenzo[ a]pyrene is readily decomposed by UV irradiation at 312 nm. The decomposed product was identified as 3-nitrobenzo[ a]pyrene-6-quinone by means of mass spectrometry and proton nuclear magnetic resonance analysis. The mutagenicity of 3,6-dinitrobenzo[ a]pyrene was 137,000 revertants/nmole for Salmonella typhimurium strain TA98, less than that for strain TA98/1,8-DNP 6, an acetyltransferase-deficient mutant, and more than that for strain YG1024, an acetyltransferase-rich mutant