Protein transport across the lung epithelial barrier
1 Departments of Medicine, Physiology, and Biophysics, Molecular Pharmacology and Toxicology, and Biomedical Engineering, Will Rogers Institute Pulmonary Research Center, Keck School of Medicine and Schools of Pharmacy and Engineering, University of Southern California, Los Angeles, California 9003...
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Published in | American journal of physiology. Lung cellular and molecular physiology Vol. 284; no. 2; pp. 247 - L259 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
United States
01.02.2003
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Subjects | |
Online Access | Get full text |
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Summary: | 1 Departments of Medicine, Physiology, and
Biophysics, Molecular Pharmacology and Toxicology, and Biomedical
Engineering, Will Rogers Institute Pulmonary Research Center, Keck
School of Medicine and Schools of Pharmacy and Engineering,
University of Southern California, Los Angeles, California 90033;
and 2 Department of Pharmacology, College of
Medicine, University of Illinois, Chicago, Illinois 60612
Alveolar lining fluid normally contains
proteins of important physiological, antioxidant, and mucosal defense
functions [such as albumin, immunoglobulin G (IgG), secretory IgA,
transferrin, and ceruloplasmin]. Because concentrations of plasma
proteins in alveolar fluid can increase in injured lungs (such as with permeability edema and inflammation), understanding how alveolar epithelium handles protein transport is needed to develop therapeutic measures to restore alveolar homeostasis. This review provides an
update on recent findings on protein transport across the alveolar epithelial barrier. The use of primary cultured rat alveolar epithelial cell monolayers (that exhibit phenotypic and morphological traits of in
vivo alveolar epithelial type I cells) has shown that albumin and IgG
are absorbed via saturable processes at rates greater than those
predicted by passive diffusional mechanisms. In contrast, secretory
component, the extracellular portion of the polymeric immunoglobulin
receptor, is secreted into alveolar fluid. Transcytosis involving
caveolae and clathrin-coated pits is likely the main route of alveolar
epithelial protein transport, although relative contributions of these
internalization steps to overall protein handling of alveolar
epithelium remain to be determined. The specific pathways and
regulatory mechanisms responsible for translocation of proteins across
lung alveolar epithelium and regulation of the cognate receptors (e.g.,
60-kDa albumin binding protein and IgG binding FcRn) expressed in
alveolar epithelium need to be elucidated.
alveolar epithelial cells; albumin; secretory component; immunoglobulin G |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-3 content type line 23 ObjectType-Review-1 |
ISSN: | 1040-0605 1522-1504 |
DOI: | 10.1152/ajplung.00235.2002 |