Rapid and efficient cell labeling with a MRI contrast agent by electroporation in the presence of protamine sulfate
To investigate various protocols for magnetic labeling of human cancer cells with ferumoxides with a view to developing an effective and fast technique for potential clinical use in MRI. Transfection methods utilizing poly- -lysine and protamine sulfate (PS), electroporation, and combination of PS w...
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Published in | Nanomedicine (London, England) Vol. 4; no. 3; pp. 305 - 315 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
England
Future Medicine Ltd
01.04.2009
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Subjects | |
Online Access | Get full text |
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Summary: | To investigate various protocols for magnetic labeling of human cancer cells with ferumoxides with a view to developing an effective and fast technique for potential clinical use in MRI.
Transfection methods utilizing poly-
-lysine and protamine sulfate (PS), electroporation, and combination of PS with electroporation were evaluated in this
study.
Although transfection was more effective in terms of uptake rates (95-–100%) and intracellular iron concentrations (4.01-–7.34 pg/cell), all transfection agents required prolonged incubation. By contrast, electroporation yielded fast labeling but with a lower efficacy (68-–75%, 1.63-–2.59 pg/cell). The addition of PS to electroporation increased the labeling efficacy (80-–91%, 2.84-–4.16 pg/cell) and protected cell viability. This combined method also resulted in the best T
*-shortening effect in the
cellular MRI.
The combined PS-–electroporation method provides a fast and efficient protocol for ferumoxide-based cellular imaging and therapeutic procedure. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 1743-5889 1748-6963 |
DOI: | 10.2217/nnm.09.6 |