Reference intervals of serum tartrate-resistant acid phosphatase type 5b activity measured with a novel assay in Japanese subjects

• Published in: Journal of bone and mineral metabolism Vol. 26; no. 3; pp. 265 - 270
• Main Authors: Nishizawa, Yoshiki, Inaba, Masaaki, Ishii, Mitsukazu, Yamashita, Hiroyuki, Miki, Takami, Goto, Hitoshi, Yamada, Sinsuke, Chaki, Osamu, Kurasawa, Kentaro, Mochizuki, Yoshiko
• Format: Journal Article
• Language: English
• Published: Japan Springer Japan 2008; Springer; Springer Nature B.V
• Subjects: Acid Phosphatase - analysis; Acid Phosphatase - blood; Adolescent; Adult; Age; Aged; Aged, 80 and over; Biological and medical sciences; Bone Density - physiology; Diseases of the osteoarticular system; Female; Humans; Isoenzymes - analysis; Isoenzymes - blood; Japan; Male; Medical sciences; Medicine; Medicine & Public Health; Menopause; Metabolic Diseases; Middle Aged; Original Article; Orthopedics; Reference Standards; Reference Values; Tartrate-Resistant Acid Phosphatase; Women; fragments absorbed immunocapture enzymatic assay (FAICEA); osteoclastic activity; tartrate-resistant acid phosphatase 5b (TRACP-5b); reference interval; Human; Enzyme; Rheumatology; Phosphoric monoester hydrolases; Esterases; Assay; Tartrate; Immunocapture; tartrate-resistant acid phosphatase 5b (TRACP- 5b); Hydrolases; Serum; Acid phosphatase
• ISSN: 0914-8779; 1435-5604
• DOI: 10.1007/s00774-007-0826-0

Among the isotypes of serum tartrate-resistant acid phosphatase (TRACP), only type 5b (TRACP-5b) is derived from osteoclasts, and it is necessary to develop an assay specific for this TRACP-5b for evaluation of osteoclastic activity. Recently, a novel assay system for TRACP-5b called the fragments absorbed immunocapture enzymatic assay (FAICEA) has been developed. With two unique monoclonal antibodies, one that is highly specific for TRACP-5b and another which absorbs inactive TRACP-5b fragments that interfere with measuring active TRACP-5b, this assay provides correct measurement of TRACP-5b activity in the serum without interference by the inactive fragments of TRACP-5b and other isotypes of TRACP, especially TRACP-5a. To study the reference data of Japanese subjects, we measured TRACP-5b activity in the serum of 320 men (age, 20–82 years) and 466 women [315 premenopausal (age, 18–55 years) and 151 postmenopausal (age, 45–77 years)] with this novel assay. In men, serum TRACP-5b activity did not vary significantly with age. The postmenopausal women had significantly higher serum TRACP-5b activity than the premenopausal women. The reference intervals (logarithmic mean ±1.96 SD) for men, premenopausal women, and postmenopausal women were 1.7–5.9 U/l, 1.2–4.4 U/l, and 2.5–7.6 U/l, respectively.