Phylogenetic structure of bacterial assemblages co-occurring with Ostreopsis cf. ovata bloom

• Published in: Harmful algae Vol. 55; pp. 259 - 271
• Main Authors: Vanucci, Silvana, Guidi, Flavio, Pistocchi, Rossella, Long, Richard A.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.05.2016
• Subjects: Actinobacteria; algal blooms; coasts; Cyanobacteria; Dinophyceae; environmental health; epiphytes; Erythrobacter; Flavobacteria; gamma-Proteobacteria; genes; HAB-associated bacteria; Haliea; humans; Jannaschia; Marine; microalgae; Ostreopsis; phylogeny; phylotype; Proteobacteria; ribosomal RNA; Ruegeria; sequence analysis; Toxic dinoflagellate; toxicity; Adriatic Sea; MED, Adriatic Sea; MED; Ostreopsis; Toxic dinoflagellate; HAB-associated bacteria; Ruegeria; Haliea; Jannaschia
• ISSN: 1568-9883; 1878-1470
• DOI: 10.1016/j.hal.2016.04.003

Extensive blooms of the toxic epiphytic/benthic dinoflagellate Ostreopsis cf. ovata are being reported with increasing frequency and spatial distribution in temperate coastal regions including the Mediterranean. These blooms are of human and environmental health concern due to the production of isobaric palytoxin and a wide range of ovatoxins by Ostreopsis cf. ovata. Bacterial-microalgal interactions are important regulators in algal bloom dynamics and potentially toxin dynamics. This study investigated the bacterial assemblages co-occurring with O. cf. ovata (OA) and from ambient seawaters (SW) during the early and peak phases of bloom development in NW Adriatic Sea. Fractions of the bacterial assemblages co-occurring with O. cf. ovata (OA) and more closely associated to the mucilage layer (LA) embedding O. cf. ovata cells were also reported. In total, 14 bacterial phyla were detected by targeted 454 pyrosequencing of the 16S rRNA gene. The dominant bacterial phyla in the OA assemblages were Proteobacteria and Bacteroidetes; while at the class level, Alphaproteobacteria were the most abundant (83 and 66%, relative abundance, early and peak bloom phases), followed by Flavobacteria (7 and 19%, early and peak phases). Actinobacteria and Cyanobacteria were of minor importance (<5% of the relative bacterial abundance each). Gammaproteobacteria showed a notably presence in OA assemblage only at the early phase of the bloom (genus Haliea, 13%). The Alphaproteobacteria were predominately composed by the genera Ruegeria, Jannaschia and Erythrobacter which represented about half of the total phylotypes’ contribution of OA at both early and peak phases of the O. cf. ovata bloom, suggesting interactions between this consortium and the microalga. Moreover, the highest contribution of Ruegeria (30% of the total phylotypes) was observed at the early phase of the bloom in LA assemblage. Microbial assemblages associated with the ambient seawaters while being also dominated by Alphaproteobacteria and Flavobacteria were partially distinct from those associated with O. cf. ovata due to the presence of genera almost not retrieved in the latter assemblages.