Ectopic expression of fungal EcGDH improves nitrogen assimilation and grain yield in rice

• Published in: Journal of integrative plant biology Vol. 60; no. 2; pp. 85 - 88
• Main Authors: Tang, Dongying, Peng, Yuchong, Lin, Jianzhong, Du, Changqing, Yang, Yuanzhu, Wang, Dan, Liu, Cong, Yan, Lu, Zhao, Xiaoying, Li, Xia, Chen, Liangbi, Liu, Xuanming
• Format: Journal Article
• Language: English
• Published: China (Republic : 1949- ) Wiley Subscription Services, Inc 01.02.2018
• Subjects: Ammonium; Assimilation; Biological assimilation; Crop yield; Ectopic expression; Ectopic Gene Expression; Eurotium; Eurotium - enzymology; Field tests; fungi; gene overexpression; Glutamate Dehydrogenase - genetics; Glutamate Dehydrogenase - metabolism; Grain; grain yield; Hydroponics; NADP; NADP (coenzyme); Nitrogen; Nitrogen - metabolism; Nitrogen - pharmacology; Oryza; Oryza - drug effects; Oryza - genetics; Oryza - metabolism; Oryza sativa; Plants, Genetically Modified; Rice; Seedlings - drug effects; Seedlings - genetics; Seeds - drug effects; Seeds - growth & development; Transgenic plants; 氮吸收效率;谷物产量;米饭;真菌;转基因;有机体;脱氢酶;植物
• ISSN: 1672-9072; 1744-7909; 1744-7909
• DOI: 10.1111/jipb.12519

NADP（H）-dependent glutamate dehydrogenases（GDH） in lower organisms have stronger ammonium affinity than those in higher plants. Here we report that transgenic rice overexpressing the EcGDH from Eurotium cheralieri exhibited significantly enhanced aminating activities. Hydroponic and field tests showed that nitrogen assimilation efficiency and grain yields were markedly increased in these transgenic plants, especially at the low nitrogen conditions.These results suggest that EcGDH may have potential to be used to improve nitrogen assimilation and grain yield in rice.