Inhibition of DNAse activity in PFGE analysis of DNA from Campylobacter jejuni

DNAse-positive strains of Campylobacter jejuni degrade their chromosomal DNA during standard preparative procedures before pulsed-field gel electrophoresis (PFGE). A simple method for inactivation of this DNAse activity is described. Formaldehyde fixation of the bacterial cells resulted in the prese...

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Bibliographic Details
Published inLetters in applied microbiology Vol. 19; no. 5; pp. 357 - 358
Main Authors Gibson, J.R, Sutherland, K, Owen, R.J
Format Journal Article
LanguageEnglish
Published England 01.11.1994
Subjects
Campylobacter jejuni
Campylobacter jejuni - genetics
deoxyribonuclease I
Deoxyribonucleases - antagonists & inhibitors
Deoxyribonucleases - metabolism
DNA
DNA, Bacterial - analysis
Electrophoresis, Gel, Pulsed-Field
enzyme activity
formaldehyde
inhibition
pulsed field electrophoresis
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Summary:DNAse-positive strains of Campylobacter jejuni degrade their chromosomal DNA during standard preparative procedures before pulsed-field gel electrophoresis (PFGE). A simple method for inactivation of this DNAse activity is described. Formaldehyde fixation of the bacterial cells resulted in the preservation of the DNA in a state suitable for restriction digestion and subsequent electrophoretic analysis.
ISSN:0266-8254
1472-765X
DOI:10.1111/j.1472-765X.1994.tb00474.x