Development of a novel carrier optimized for cell sheet transplantation

• Published in: Biomatter (Austin, TX) Vol. 5; no. 1; p. e1027846
• Main Authors: Amagai, Yosuke, Karasawa, Kaoru, Kyungsook, Jung, Matsuda, Akira, Kojima, Masanori, Watanabe, Jun, Hibi, Toyoji, Matsuda, Hiroshi, Tanaka, Akane
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis 2015
• Subjects: Animals; Carrier; Cell Culture Techniques - instrumentation; cell sheet; Cell Transplantation - instrumentation; Cell Transplantation - methods; Cells, Cultured; cornea; Corneal Injuries - surgery; Corneal Transplantation - instrumentation; Corneal Transplantation - methods; Dogs; Lactic Acid - metabolism; Male; Membranes, Artificial; poly(lactic acid); Polyesters; Polymers - metabolism; Rats; Regenerative Medicine - instrumentation; Short Communication; Skin - injuries; Skin Transplantation - instrumentation; Skin Transplantation - methods; Tissue Engineering - instrumentation; transplantation; PBS, phosphate-buffered saline; cornea; α-MEM, α-minimum essential medium; FBS, fetal bovine serum; cell sheet; transplantation; Carrier; poly(lactic acid)
• ISSN: 2159-2535; 2159-2527; 2159-2535
• DOI: 10.1080/21592535.2015.1027846

Tissue engineering is a rapidly advancing technology in the field of regenerative medicine. For the transplantation of cell sheets, a carrier must maintain the shape of a cell sheet from a culture dish to affected sites as well as release the sheet easily onto the lesion. In this study, we examined the utility of a novel, poly(lactic acid)-based carrier for cell sheets transplantation to the cornea of dogs and the skin of rats. The poly(lactic acid)-based carrier easily picked a cell sheet up from the dish, fit to the shape of the transplantation sites, and saved time for cell sheets detachment comparing to a conventional carrier. Thus, the poly(lactic acid)-based carrier would be useful for easy cell sheet transplantations.