Normal or Retrovirus-Infected Cultured Chicken Embryo Cells Express the 48,000 D Age-Related Antigen Characteristic of Embryonic Chicken Erythrocytes

The surface of normal or retrovirus-infected chick embryo cells was labelled with 125I using lactoperoxidase. The solubilized membrane material was allowed to react with antisera raised in rabbits to cultured chick embryo cells or to the membranes of embryonic or adult chicken erythrocytes. Analysis...

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Bibliographic Details
Published inDifferentiation (London) Vol. 18; no. 1; pp. 175 - 178
Main Authors PERISIC, O., BIQUARD, J.M., BLANCHET, J.P., GREENLAND, T.B., KRSMANOVIC, V.
Format Journal Article
LanguageEnglish
Published Oxford, UK Elsevier B.V 01.01.1981
Blackwell Publishing Ltd
Subjects
Animals
Antibody Specificity
Antigen-Antibody Reactions
Antigens, Surface - analysis
Antigens, Surface - immunology
Cells, Cultured
Chick Embryo - immunology
Erythrocytes - immunology
Membrane Proteins - immunology
Molecular Weight
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Summary:The surface of normal or retrovirus-infected chick embryo cells was labelled with 125I using lactoperoxidase. The solubilized membrane material was allowed to react with antisera raised in rabbits to cultured chick embryo cells or to the membranes of embryonic or adult chicken erythrocytes. Analysis of the immunoprecipitates shows that chicken embryo cells not of erythropoietic origin express on their surface membrane an antigenic polypeptide of mol. wt. 48,000 daltons (D), which appears to be identical with that expressed on embryonic chicken erythrocytes.
Bibliography:L
L50
Biological Laboratory 090, Boris Kidrich Institut, P.O. Box 522, Belgrade, Yugoslavia
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0301-4681
1432-0436
DOI:10.1111/j.1432-0436.1981.tb01119.x