Complete mitochondrial DNA sequence of the Japanese flying fish Cypselurus hiraii
In this study, to develop a technique that enables authentication of processed seafood, the complete nucleotide sequence of the mitochondrial genome for the Japanese flying fish Cypselurus hiraii was determined. Three segments spanning the entire genome were amplified using polymerase chain reaction...
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Published in | Fisheries science Vol. 71; no. 4; pp. 914 - 923 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Melbourne, Australia
Blackwell Science Pty
01.08.2005
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | In this study, to develop a technique that enables authentication of processed seafood, the complete nucleotide sequence of the mitochondrial genome for the Japanese flying fish Cypselurus hiraii was determined. Three segments spanning the entire genome were amplified using polymerase chain reaction, and products were subsequently used as templates for direct sequencing with 60 primers. The genome (16 528 base pairs) was found to contain the same 37 genes (two ribosomal RNA, 22 transfer RNA, and 13 protein-coding genes) as those found in other vertebrate mitochondrial genomes, with the gene order being identical to that typical of vertebrates. A major noncoding region between the tRNAPro and tRNAphe genes (868 base pairs) appears to be the control (Dloop) region, as it has several conserved blocks characteristic of control regions. |
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Bibliography: | Q04 L10 2006001520 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1 |
ISSN: | 0919-9268 1444-2906 |
DOI: | 10.1111/j.1444-2906.2005.01045.x |