Production and purification of agarase from a marine agarolytic bacterium Agarivorans sp. HZ105

• Published in: Journal of applied microbiology Vol. 106; no. 1; pp. 181 - 190
• Main Authors: Hu, Z, Lin, B.-K, Xu, Y, Zhong, M.Q, Liu, G.-M
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 2009; Blackwell Publishing Ltd; Blackwell
• Subjects: agarase; Agarivorans HZ105; Agarivorans sp. HZ105; Alteromonadaceae - enzymology; Alteromonadaceae - genetics; Alteromonadaceae - isolation & purification; Bacteria; Biological and medical sciences; Electrophoresis, Polyacrylamide Gel; Enzyme Stability; Fundamental and applied biological sciences. Psychology; Geologic Sediments - microbiology; Glycoside Hydrolases - chemistry; Glycoside Hydrolases - isolation & purification; Hydrogen-Ion Concentration; identification; isolation; Microbiology; Phenotype; purification; RNA, Ribosomal, 16S - genetics; RNA, Ribosomal, 16S - isolation & purification; Sodium Chloride; Purification; Enzyme; Applied microbiology; MS; Identification; Glycosylases; Marine environment; Glycosidases; Production; Agarase; Hydrolases; Bacteria; Isolation; Agarivorans sp. HZ105
• ISSN: 1364-5072; 1365-2672
• DOI: 10.1111/j.1365-2672.2008.03990.x

Isolation and characterization of an agarase-producing bacterium Agarivorans sp. HZ105. An agarase-producing bacterium strain HZ105 had been isolated from marine sediment sample. Based on phylogenetic analysis of the 16S rRNA gene sequence and phenotypic analysis, as well as biochemical analyses, this strain was named Agarivorans sp. HZ105. Effect of pH, NaCl on the growth and agarase production of strain HZ105 was studied. Strain HZ105 produced three extracellular agarases which were purified to homogeneity from bands in the PAGE gel. Two agarases of these three had a molecular mass of 54, 58 kDa, respectively. And the MS and MS/MS spectra were used to identify the agarases. The MS spectra result showed that the agarases of strain HZ105 should be beta-agarase and belong to the family 50 of glycosyl hydrolases. The agarases could keep stable activity at room temperature. The strain HZ105 was useful to produce stable agarases. The solution produced by agar's degradation in the agar plates was first reported to be used for purification of agarase. Agarases were purified to homogeneity directly from the PAGE gel without stained by Coomassie brilliant blue.