Effect of leukotrienes, bradykinin and calcium ionophore (A 23187) on bovine endothelial cells: Release of prostacyclin

• Published in: Prostaglandins Vol. 31; no. 1; pp. 157 - 166
• Main Authors: Clark, Mike A., Littlejohn, David, Mong, Seymour, Crooke, Stanley T.
• Format: Journal Article
• Language: English
• Published: Stoneham, MA Elsevier Inc 1986; Butterworth-Heinemann
• Subjects: 6-Ketoprostaglandin F1 alpha - metabolism; Animals; Arachidonic Acid; Arachidonic Acids - pharmacology; Biological and medical sciences; Bradykinin - pharmacology; Calcimycin - pharmacology; Cattle; Cell Line; Endothelium - drug effects; Endothelium - metabolism; Epoprostenol - biosynthesis; Fundamental and applied biological sciences. Psychology; Kinetics; Prostaglandins. Arachidonic acid metabolites; SRS-A - pharmacology; Vertebrates: endocrinology; Calcium; Prostaglandin I2; Secretion; Arachidonic acid derivatives; Peptide hormone; Inorganic element; Biological activity; Endothelium; Leukotriene; Vertebrata; Regulation(control); Mammalia; Membrane transport; Bradykinin; Blood vessel; Artiodactyla; Circulatory system; Beef; Mechanism of action; Ungulata
• ISSN: 0090-6980
• DOI: 10.1016/0090-6980(86)90233-9

Incubation of the bovine endothelial cell line, CPAE, with the calcium ionophore (A23187), bradykinin (BK), leukotriene D 4 (LTD 4) or leukotriene C 4 (LTC 4) resulted in concentration dependent increases in prostacyclin release measured as 6-ketoprostaglandin F 1α. The kinetics of induction of prostacyclin synthesis differed among the agents studied. Statistically significant increases in prostacyclin were observed one minute after treatment with A23187, at slightly longer times with bradykinin and after approximately three minutes with the leukotrienes. Two other leukotrienes were tested. Both leukotriene B 4 and leukotriene E 4 (LTE 4) were inactive at con- centrations up to 10 μM. The induction of prostacyclin synthesis by LTC 4 and LTD 4 was inhibited by cycloheximide and actinomycin-D. The effect of BK was inhibited by cycloheximide but not by actinomycin-D. Induction by A23107 was not inhibited by either actinomycin-D or cycloheximide. The results suggest that these agents induced the increases in prostacyclin synthesis by different mechanisms.