Suppression of mast cell colony formation by a low molecular weight fraction of fetal calf bone marrow extract

• Published in: Leukemia Research Vol. 13; no. 12; pp. 1085 - 1089
• Main Authors: Lenfant, Maryse, Sotty, Dominique, Wdzieczak-Bakala, Joanna, Itoh, Katsuhiko, Shirata, Katsutoshi, Sugimoto, Kenkichi, Mori, Kazuhiro J.
• Format: Book Review Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 1989; Elsevier Science
• Subjects: Animals; Biological and medical sciences; Bone Marrow - physiology; Cattle; Cell differentiation, maturation, development, hematopoiesis; Cell Division - drug effects; Cell physiology; Female; Fundamental and applied biological sciences. Psychology; Haemopoiesis; IL-3; Inhibitor; Interleukin-3 - pharmacology; Mast Cell; Mast Cells - drug effects; Mast Cells - physiology; Mice; Mice, Inbred C3H; Molecular and cellular biology; Stem Cells - drug effects; Tissue Extracts - pharmacology; STIL-3-CM, conditioned medium from STIL-3; FCBM, fetal calf bone marrow; Mast Cell; Inhibitor; IL-3; Haemopoiesis; GM-CSF, granulocyte/macrophage colony-stimulating factor; CFU-S, colony forming unit-spleen; IL-3, interleukin-3; FCS, fetal calf serum; Cell proliferation; Bovine; Rodentia; Cytokine; Colony forming cell; Tissue extract; Vertebrata; Mammalia; Cell line; Mouse; Hematopoiesis; Interleukin 3; Bone marrow; Fetus; Mast cell; Artiodactyla; Antagonist; Ungulata
• ISSN: 0145-2126; 1873-5835
• DOI: 10.1016/0145-2126(89)90153-7

A semi-purified fraction extracted from fetal calf bone marrow was previously shown to contain a tetrapeptide N-Ac-Ser-Asp-Lys-Pro which inhibits in mice, hematopoietic stem cell entry into the cell cycle. This peptide however, did not exhibit any effect on either IL-3 nor GM-CSF dependent cell growth. We report herein that a semi-purified fraction also contains another activity which is antagonistic to IL-3. Addition of the fraction in vitro decreased IL-3 dependent mast cell colony formation. Growth of IL-3 dependent cell lines (DA-1 and FDC-P2) was also suppressed. No effect was observed in the same dose range on granulocyte-macrophage colony formation nor on IL-3 independent cell growth.