A quadruplet electrochemical platform for ultrasensitive and simultaneous detection of ascorbic acid, dopamine, uric acid and acetaminophen based on a ferrocene derivative functional Au NPs/carbon dots nanocomposite and graphene

• Published in: Analytica chimica acta Vol. 903; pp. 69 - 80
• Main Authors: Yang, Liuqing, Huang, Na, Lu, Qiujun, Liu, Meiling, Li, Haitao, Zhang, Youyu, Yao, Shouzhuo
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.01.2016
• Subjects: acetaminophen; Acetaminophen - analysis; analytical chemistry; ascorbic acid; Ascorbic Acid - analysis; Au/C NC; detection limit; dielectric spectroscopy; dopamine; Dopamine - analysis; electrochemistry; Ferrous Compounds - chemistry; Fourier transform infrared spectroscopy; glassy carbon electrode; gold; Graphene; Humans; Limit of Detection; Metallocenes; Nanocomposites; nanogold; oxidation; Quadruplet detection; reducing agents; Reproducibility of Results; scanning electron microscopy; Sensitivity and Specificity; Signal amplification; Simultaneous determination; thiols; transmission electron microscopy; uric acid; Uric Acid - analysis; Simultaneous determination; Au/C NC; Graphene; Signal amplification; Quadruplet detection
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/j.aca.2015.11.021

In this work, a new nanomaterial of thiol functional ferrocene derivative (Fc-SH) stabilized Au NPs/carbon dots nanocomposite (Au/C NC) coupling with graphene modified glassy carbon electrode (Fc-S-Au/C NC/graphene/GCE) was fabricated to serve as a quadruplet detection platform for ultrasensitive and simultaneous determination of ascorbic acid (AA), dopamine (DA), uric acid (UA) and acetaminophen (AC). The Au/C NC was synthesized by adding HAuCl4 into carbon nanodots solution without using any additional reductant and stabilizing agent. Then the Fc-SH was utilized as the protective and capping agent to modify the Au/C NC. Transmission electron microscopy (TEM), UV–Vis, Fourier-transform infrared (FT-IR), scanning electron microscopy (SEM), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) were adopted to characterize the morphology and electrochemical properties of the materials and the electrodes. The Fc-S-Au/C NC/graphene/GCE exhibits a synergistic catalytic and amplification effects towards oxidation of AA, DA, UA and AC owing to the existence of the nanomaterial and electron mediator. When simultaneous detection of AA, DA, UA and AC, the oxidation peak potentials of the four compounds on the electrode can be well separated and the peak currents were linearly dependent on their concentrations. The quadruplet detection platform shows excellent linear range and ultrasensitive response to the four components, the detection limits were estimated to be as low as 1.00, 0.05, 0.12 and 0.10 μM (S/N = 3), and the modified electrode exhibits excellent stability and reproducibility. The proposed electrode has been successfully applied to detect of these four analytes in real samples with satisfactory results. [Display omitted] •A quadruplet detection platform for ultrasensitive and simultaneous determination of AA, DA, UA and AC was constructed.•The platform was based on the composite containing Au/C NC, graphene and a ferrocene derivative.•It is the first time simultaneously uses Au/C NC and electron mediator in electrochemical detection purpose.•The platform is simple, convenient and effective to detect the four species in the complex samples with higher sensitivity.