Serious complications in gene-expression studies with stress perturbation An example of UV-exposed p53-mutant mouse embryonic fibroblasts

Reanalysis of our UV study of p53-mutant mouse embryonic fibroblasts revealed an intriguing orchestration of massive transcriptome responses. However, close scrutiny of the data uncovered an affected mRNA/rRNA ratio, effectively inhibiting valid data analysis. UV-dose range-finding showed low-dose U...

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Published inTranscription Vol. 1; no. 3; pp. 159 - 164
Main Authors Bruning, Oskar, Yuan, Xiaolian, Rodenburg, Wendy, Bruins, Wendy, van Oostrom, Conny T., Rauwerda, Han, Wittink, Floyd R.A., Jonker, Martijs J., de Vries, Annemieke, Breit, Timo M.
Format Journal Article
LanguageEnglish
Published United States Taylor & Francis 01.11.2010
Landes Bioscience
Subjects
Binding
Biology
Bioscience
Calcium
Cancer
Cell
Cycle
Landes
Organogenesis
Point-of-View
Proteins
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Summary:Reanalysis of our UV study of p53-mutant mouse embryonic fibroblasts revealed an intriguing orchestration of massive transcriptome responses. However, close scrutiny of the data uncovered an affected mRNA/rRNA ratio, effectively inhibiting valid data analysis. UV-dose range-finding showed low-dose UV specific- and high-dose stress-related responses, which represent a plea for UV dose range-finding in experimental design.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:2154-1264
2154-1272
DOI:10.4161/trns.1.3.13487