Solving the Problem of Ambiguous Paralogy for Marker Loci: Microsatellite Markers with Diploid Inheritance in Allohexaploid Mercurialis annua (Euphorbiaceae)

Mercurialis annua is a wind-pollinated annual showing a remarkable sexual-system variation, with hexaploid populations being either monoecious or androdioecious. Hexaploid M. annua is most likely a product of hybridization between diploid M. huetii and tetraploid M. annua; therefore, we developed mi...

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Published inThe Journal of heredity Vol. 101; no. 4; pp. 504 - 511
Main Authors Korbecka, Grażyna, Rymer, Paul D, Harris, Stephen A, Pannell, John R
Format Journal Article
LanguageEnglish
Published United States The American Genetic Association 01.07.2010
Oxford University Press
Oxford Publishing Limited (England)
Subjects
allopolyploid
androdioecy
Chromosome Mapping
Diploidy
DNA, Plant - metabolism
Euphorbiaceae
Euphorbiaceae - genetics
Gene loci
Genetic Linkage
Genetic Loci
Genetic markers
Genome, Plant
Heredity
mating system
Mercurialis annua
Microorganisms
microsatellite
Microsatellite Repeats - genetics
Minisatellite Repeats
Polymerase Chain Reaction
Sequence Analysis
SSR markers
Studies
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Summary:Mercurialis annua is a wind-pollinated annual showing a remarkable sexual-system variation, with hexaploid populations being either monoecious or androdioecious. Hexaploid M. annua is most likely a product of hybridization between diploid M. huetii and tetraploid M. annua; therefore, we developed microsatellite loci by isolating simple sequence repeat (SSR) sequences from the diploid progenitor, cross-amplification tests in M. huetii/M. annua species complex followed by selection of loci amplifying only in M. huetii and hexaploid M. annua, and testing polymorphism in 1 hexaploid population. This protocol resulted in 10 unlinked, polymorphic loci amplifying 4-10 alleles per locus. Due to specific amplification of the diploid part of the genome originating from M. huetii, these loci produce codominantly scored, diploid data for allohexaploid species, thereby simplifying data collection and subsequent analyses. Sequencing of the hexaploid polymerase chain reaction product for all 10 loci and aligning it with M. huetii SSR library sequence confirmed orthology of the characterized loci. Inheritance tests in 4 hexaploid crosses confirmed diploid Mendelian segregation of the new loci.
Bibliography:These authors contributed almost equally.
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ISSN:0022-1503
1465-7333
DOI:10.1093/jhered/esq026