Effect of octenylsuccinic anhydride modification on β-amylolysis of starch

• Published in: Carbohydrate polymers Vol. 97; no. 1; pp. 9 - 17
• Main Authors: Sweedman, Michael C., Hasjim, Jovin, Tizzotti, Morgan J., Schäfer, Christian, Gilbert, Robert G.
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 14.08.2013; Elsevier
• Subjects: Applied sciences; beta-amylase; beta-Amylase - metabolism; Beta-amylase hydrolysis; Biological and medical sciences; Exact sciences and technology; Food industries; Fundamental and applied biological sciences. Psychology; gelatinization; Hydrolysis; Kinetics; molecular weight; Natural polymers; Nuclear magnetic resonance; Octenylsuccinic anhydride; Physicochemistry of polymers; Size exclusion chromatography; Sorghum - chemistry; Starch; Starch - chemistry; Starch and polysaccharides; Starch and starchy product industries; Succinates - chemistry; waxy corn; Zea mays - chemistry; Size exclusion chromatography; Nuclear magnetic resonance; Starch; Octenylsuccinic anhydride; Beta-amylase hydrolysis; Enzyme; Experimental study; Lateral group; Glycosylases; β-Amylase; Reactivity; Glycosidases; Enzymatic digestion; Hydrolases; Oside polymer; Hydrophobic group; Kinetics; Starch derivative
• ISSN: 0144-8617; 1879-1344; 1879-1344
• DOI: 10.1016/j.carbpol.2013.04.041

•Starches modified with octyl succinic anhydride are common food additives.•Sorghum and waxy maize starches were modified with OSA and subject to β-amylolysis.•This is a way to improve functional properties by reducing molecular size.•Presence of OS groups shows minor inhibitory effects on β-amylolysis. The effects of octenylsuccinic anhydride (OSA) modification of waxy maize and sorghum starches on subsequent β-amylolysis are examined. Hydrolysis with β-amylase is a method by which OSA starches may be structurally modified for industrial purposes. The hydrolysis of both granular and gelatinised forms of both starches follows first-order kinetics regardless of the OSA used as a percent of starch mass (0–24%). The highest hydrolysis rate coefficients for granular starches are at modification with 6% OSA/starch. The largest molecular sizes of β-amylase hydrolysed OSA-modified gelatinised starches are found at modification with 24% OSA/starch. The results suggest that octenylsuccinyl groups have an action-blocking effect on β-amylolysis of gelatinised starch, but the effect of semi-crystalline granular structure is more pronounced than that of OSA modification. Hence β-amylolysis can be used under appropriate conditions to modify the structure of gelatinised OSA-modified starches.