Immunoreactivity of a G protein-coupled l-DOPA receptor GPR143, in Lewy bodies

• Published in: Neuroscience research Vol. 148; pp. 49 - 53
• Main Authors: Goshima, Yoshio, Watanabe, Shuya, Seki, Erika, Koga, Motokazu, Masukawa, Daiki, Nakamura, Fumio, Komori, Takashi, Arai, Nobutaka
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier B.V 01.11.2019
• Subjects: alpha-Synuclein - metabolism; Bodies; Brain; Dopamine - metabolism; Eye Proteins - metabolism; GPR143; HEK293 Cells; Humans; l-DOPA; Lewy; Lewy Bodies - metabolism; Membrane Glycoproteins - metabolism; Parkinson Disease - metabolism; Parkinson’s disease; Receptors, G-Protein-Coupled; Receptors, Neurotransmitter; Substantia Nigra - metabolism; α-Synuclein; PBS; α-Synuclein; RT; GPR143; PD; l-DOPA; SNpc; SN; Bodies; Parkinson’s disease; Lewy
• ISSN: 0168-0102; 1872-8111; 1872-8111
• DOI: 10.1016/j.neures.2018.12.004

•We generated an antibody raised against human GPR143, a l-DOPA receptor.•Immunoreactive signals of GPR143 are localized in Lewy bodies.•GPR143 is colocalized with phosphorylated α-synuclein at Ser129. l-3,4-Dihydroxyphenylalanine (l-DOPA) has been believed to be an inert amino acid precursor of dopamine, and is the most effective therapeutic agent in Parkinson’s disease (PD). We proposed l-DOPA as a neurotransmitter in the central nervous system. Recently, the ocular albinism 1 gene product, OA1/GPR143 (GPR143), was identified as a receptor for l-DOPA. In this study, we examined by generating anti-human GPR143 antibody, the localization of GPR143-immunoreactive signals in the brains from control and PD subjects. GPR143-immunoreactive signals were detected throughout the entire midbrain including substantia nigra pars compacta. In the PD brains, we found that GPR143-immunoreactive signals were detected in Lewy bodies and were colocalized with immunoreactive signals with anti-human Ser129 phosphorylated α-synuclein antibody. Although the significance of its occurrence in the inclusion bodies is unknown, our finding suggests possible implications of GPR143 in PD.