Investigating the reactivities of a polyketide synthase module through fluorescent click chemistry

A method for monitoring in vitro polyketide synthesis has been developed whereby nonchromophoric polyketide products are made brightly fluorescent in a simple, rapid, inexpensive, and bioorthogonal manner through CuAAC with a sulforhodamine B azide derivative.

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Bibliographic Details
Published inChemical communications (Cambridge, England) Vol. 50; no. 40; pp. 5276 - 5278
Main Authors Hughes, Amanda Jane, Tibby, Matthew R., Wagner, Drew T., Brantley, Johnathan N., Keatinge-Clay, Adrian T.
Format Journal Article
LanguageEnglish
Published CAMBRIDGE Royal Soc Chemistry 25.05.2014
Subjects
Azides - chemistry
Catalysis
Chemistry
Chemistry, Multidisciplinary
Click Chemistry
Copper - chemistry
Derivatives
Fluorescence
Fluorescent Dyes
Modules
Monitoring
Physical Sciences
Polyketide Synthases - metabolism
Polyketides - metabolism
Rhodamines
Science & Technology
Synthesis
Synthesis (chemistry)
CHIRAL BUILDING-BLOCKS
MECHANISM
SELECTIVITY
BIOSYNTHESIS
BIOCATALYSIS
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Summary:A method for monitoring in vitro polyketide synthesis has been developed whereby nonchromophoric polyketide products are made brightly fluorescent in a simple, rapid, inexpensive, and bioorthogonal manner through CuAAC with a sulforhodamine B azide derivative.
Bibliography:NIH RePORTER
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:1359-7345
1364-548X
DOI:10.1039/c3cc47513a