Human chorionic gonadotropin causes an estrogen-mediated induction of rat ovarian carbonyl reductase

We earlier reported that human chorionic gonadotropin (hCG) stimulates rat ovarian carbonyl reductase (CR) activity and content, and that estrogen enhances the stimulatory effect. The present study was performed to determine the mode of action of the gonadotropin. Cycloheximide (CHX) and actinomycin...

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Bibliographic Details
Published inLife sciences (1973) Vol. 51; no. 11; p. 817
Main Authors Inazu, N, Inaba, N, Satoh, T, Fujii, T
Format Journal Article
LanguageEnglish
Published Netherlands 1992
Subjects
Alcohol Oxidoreductases - biosynthesis
Alcohol Oxidoreductases - drug effects
Animals
Chorionic Gonadotropin - pharmacology
Cycloheximide - pharmacology
Dactinomycin - pharmacology
DNA-Directed RNA Polymerases - antagonists & inhibitors
Enzyme Induction
Estrogens - pharmacology
Female
Humans
Organ Size - drug effects
Ovary - anatomy & histology
Ovary - drug effects
Ovary - enzymology
Protein Biosynthesis
Rats
Rats, Inbred WKY
Uterus - anatomy & histology
Uterus - drug effects
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Summary:We earlier reported that human chorionic gonadotropin (hCG) stimulates rat ovarian carbonyl reductase (CR) activity and content, and that estrogen enhances the stimulatory effect. The present study was performed to determine the mode of action of the gonadotropin. Cycloheximide (CHX) and actinomycin D (AD) were given to estradiol-pretreated immature rats 6 h before hCG treatment. The enzyme activity was measured with three substrates, and the enzyme content was determined by the method of Western-blot analysis using anti-rat ovarian CR anti-serum as the first antibody. Both protein inhibitors significantly prevented hCG from increasing the enzyme activity and content in estradiol-pretreated ovary. These results indicate that rat ovarian CR is induced by LH via the action of estrogen.
ISSN:0024-3205
DOI:10.1016/0024-3205(92)90608-R