Development of novel amphiphilic magnetic molecularly imprinted polymers compatible with biological fluids for solid phase extraction and physicochemical behavior study

• Published in: Journal of Chromatography A Vol. 1317; pp. 110 - 120
• Main Authors: Dramou, Pierre, Zuo, Pengli, He, Hua, Pham-Huy, Lien Ai, Zou, Wenyue, Xiao, Deli, Pham-Huy, Chuong
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 22.11.2013
• Subjects: adsorption; Amphiphilic magnetic-imprinted polymers; Aqueous media; Biological; Biological fluids; blood serum; Chemical Phenomena; chromatography; clinical trials; dispersibility; drug carriers; Extraction; Fluid dynamics; Fluid flow; Fluids; Fluoroquinolones - blood; Fluoroquinolones - urine; Fourier transform infrared spectroscopy; Hexanes; Humans; Hydrophobic and Hydrophilic Interactions; Magnetite Nanoparticles - chemistry; molecular imprinting; Molecular Imprinting - methods; monitoring; nanomaterials; Nanostructure; Oleic Acid; Physicochemical behavior study; Polymers; Scanning electron microscopy; Solid phase extraction; Solid Phase Extraction - methods; Solid phases; solubility; solvents; transmission electron microscopy; urine; washing; Water; X-radiation; X-ray diffraction; Amphiphilic magnetic-imprinted polymers; Solid phase extraction; Aqueous media; Biological fluids; Physicochemical behavior study
• ISSN: 0021-9673; 1873-3778
• DOI: 10.1016/j.chroma.2013.07.075

•Novel amphiphilic magnetic imprinted polymer (M-MIP) has been developed.•Physicochemical analysis demonstrating amphiphilicity were done for the first time.•M-MIP prepared in organic solvent found to be compatible in aqueous environment.•Compatible with biological fluids, suitability as sorbent for extraction was shown.•High recovery of the therapeutic agent (GTFX) from biological fluids was obtained. In the present work, a novel amphiphilic magnetic molecularly imprinted polymer (M-MIP) has been synthesized by a simple non covalent method for the loading of gatifloxacin (GTFX) in polar solvent. This nanomaterial used as sorbent has been applied to the solid phase extraction of GTFX in different spiked biological fluids. For the first time, studies of dispersibility and solubility behaviors with different solvents and water were performed to demonstrate amphiphilicity and also to find the better nanomaterial obtained. Fourier transform infrared (FT-IR), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and X-ray (XRD) were used to characterize the nanomaterials, and Scatchard plot analysis to demonstrate the binding kinetic. Results suggest that the dispersibility, solubility and the adsorption in water have relationships with the structure of nanomaterials prepared. The oleic acid coated on the M-MIP combined with the washing process has enhanced the amphiphilicity of the nanomaterials. The M-MIP2 showed better selectivity and adsorption behavior with imprinted efficiency higher than (2) in water, as well as in biological fluids. Moreover, no interference with constituents of blank urine and blank serum samples for solid phase extraction (SPE) was observed. Moreover, loading recovery was found higher than 95% with low RSD. The novel amphiphilic magnetic nanomaterial prepared here as sorbent is suitable for SPE of GTFX in biological fluids for therapeutic monitoring control. It could be also used as carrier in drug delivery system for experimental and clinical studies.