Nerve growth factor (NGF) restores depletions of calcitonin gene-related peptide and substance P in sensory neurons from diabetic mice in vitro

• Published in: Journal of the neurological sciences Vol. 126; no. 1; pp. 1 - 5
• Main Authors: Sango, Kazunori, Verdes, Jose M., Hikawa, Naoshi, Horie, Hidenori, Tanaka, Shun-ichi, Inoue, Shuji, Sotelo, Jose R., Takenaka, Toshifumi
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier B.V 01.10.1994; Elsevier Science
• Subjects: Animals; Associated diseases and complications; Biological and medical sciences; Calcitonin gene-related peptide; Calcitonin Gene-Related Peptide - deficiency; Calcitonin Gene-Related Peptide - metabolism; Cell Survival - drug effects; Cells, Cultured; Diabetes Mellitus, Experimental - chemically induced; Diabetes Mellitus, Experimental - complications; Diabetes Mellitus, Experimental - genetics; Diabetes Mellitus, Type 1 - metabolism; Diabetes Mellitus, Type 1 - pathology; Diabetes. Impaired glucose tolerance; Diabetic Neuropathies - pathology; Diabetic neuropathy; Dorsal root ganglion; Endocrine pancreas. Apud cells (diseases); Endocrinopathies; Female; Ganglia, Spinal - pathology; Medical sciences; Mice; Mice, Inbred C57BL; Mice, Inbred NOD; Mouse; Nerve growth factor (NGF); Nerve Growth Factors - pharmacology; Nerve Tissue Proteins - deficiency; Nerve Tissue Proteins - metabolism; Neurons, Afferent - drug effects; Neurons, Afferent - metabolism; Streptozocin; Substance P; Substance P - deficiency; Substance P - metabolism; Calcitonin gene-related peptide; Nerve growth factor (NGF); Diabetic neuropathy; Mouse; Dorsal root ganglion; Substance P; Endocrinopathy; Animal model; Nervous system diseases; Pathophysiology; Diabetes mellitus; Cytokine; Peptide hormone; Rodentia; Nerve growth factor; Peripheral neuropathy; Calcitonine gene related peptide; Neuropeptide; In vitro; Vertebrata; Experimental disease; Mammalia; Polypeptide; Animal; Sensory polyneuropathy; Growth factor
• ISSN: 0022-510X; 1878-5883
• DOI: 10.1016/0022-510X(94)90087-6

Dorsal root ganglion neurons from streptozotocin (STZ)-induced diabetic, genetic diabetic and normal mice were cultured in serum-containing media with or without nerve growth factor (NGF). The immunocytochemical analysis carried out after 1 week in culture revealed that the ratios of neurons immunoreactive to calcitonin gene-related peptide (CGRP) in NGF-free medium in the STZ-diabetic mice (average 23.2%) were significantly lower than those in the normal mice (45.1%). The ratios of neurons immunoreactive to CGRP and substance P (SP) in the NGF-free medium were also lower in the genetic diabetic mice (23.6% and 21.8%) than those in the normal ones (40.7% and 34.2%). However, treatment with NGF restored these reduced immunoreactivities in the diabetic groups in a dose-dependent manner. These results show that NGF can be effective for the diabetes-induced depletion of CGRP and SP in sensory neurons, and suggest its possible role in the prevention and improvement of diabetic sensory neuropathy.