Porcine Circovirus 3a Field Strains in Free-Living Wild Boars in Paraná State, Brazil

Porcine circovirus 3 (PCV-3) was identified in domestic pigs worldwide. Although PCV-3 has also been detected in wild boars, information regarding its circulation in this free-living animal species is scarce. To investigate PCV-3 occurrence in free-living wild boars in Brazil, 70 serum samples colle...

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Published inAnimals (Basel) Vol. 11; no. 6; p. 1634
Main Authors de Souza, Tatiana Carolina Gomes Dutra, Gava, Danielle, Schaefer, Rejane, Leme, Raquel Arruda, da Silva Porto, Gisele, Alfieri, Amauri Alcindo
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 31.05.2021
MDPI
Subjects
Animal species
Animals
circoviruses
Communication
Deoxyribonucleic acid
DNA
DNA sequencing
Domestic animals
feral pig
Genomes
Hogs
Infections
Lungs
Nucleotides
Phylogenetics
Phylogeny
Polymerase chain reaction
Pork
Proteins
Sus scrofa
Swine
Viruses
Brazil
Italy
United States > US
Spain
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Summary:Porcine circovirus 3 (PCV-3) was identified in domestic pigs worldwide. Although PCV-3 has also been detected in wild boars, information regarding its circulation in this free-living animal species is scarce. To investigate PCV-3 occurrence in free-living wild boars in Brazil, 70 serum samples collected between January 2017 and June 2019 in Paraná state, Brazil were analyzed by PCR assay. Amplicons measuring 330 bp in length were amplified in seven (10.0%) of the serum samples and confirmed to be PCV3-specific by nucleotide (nt) sequencing. As the amplified products from the serum samples yielded only intermediate levels of viral DNA, lung samples from the seven PCR-positive wild boars were also evaluated by PCR. Of these samples, five lung samples were positive and provided high levels of viral DNA. The three lung samples that presented the highest levels of viral DNA were selected for amplification and sequencing of the whole PCV-3 genome. The three full-length sequences obtained were grouped in PCV-3 clade “a”, and the sequences exhibited 100% nucleotide similarity among them. The PCV-3 field strains of this study showed nucleotide and amino acid similarities of 98.5–99.8% and 98.8–100%, respectively, with whole-genome PCV-3 sequences from around the world.
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These authors contributed equally to this study.
ISSN:2076-2615
2076-2615
DOI:10.3390/ani11061634