A Modified Method for the Development of SSR Molecular Markers Based on Redundant EST Data and Its Application in Soybean

EST-derived SSR marker has been developed in many species, but few methods of high efficiency have been reported for the exploitation of EST-SSR markers. Thus, a high efficiency method for mining millions of redundant EST data is needed. A modified method for the EST-SSR development with high effici...

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Published inJournal of Integrative Agriculture Vol. 11; no. 4; pp. 545 - 555
Main Authors ZHAO, Xue, CHANG, Wei, HAN, Ying-peng, TENG, Wei-li, LI, Wen-bin
Format Journal Article
LanguageEnglish
Published Elsevier B.V 01.04.2012
Science Press
Key Laboratory of Soybean Biology, Ministry of Education/Soybean Research Institute, Northeast Agricultural University, Harbin 150030,P.R. China
Elsevier
Subjects
EST-SSR
EST-SSR soybean polymorphism genetic mapping in silico mapping
genetic mapping
in silico mapping
polymorphism
soybean
SSR分子标记
ST数
冗余数据
大豆
应用
开发
改性方法
遗传图谱构建
soybean
in silico mapping
polymorphism
genetic mapping
EST-SSR
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Summary:EST-derived SSR marker has been developed in many species, but few methods of high efficiency have been reported for the exploitation of EST-SSR markers. Thus, a high efficiency method for mining millions of redundant EST data is needed. A modified method for the EST-SSR development with high efficiency was established based on the redundant EST data of soybean in this study. The method achieved its function through classifying ESTs according to the same SSR motif and detected candidate loci with redundant sequences. In this study, a total of 80 polymorphic EST-SSR markers of soybean were developed, 50 of them were exploited by this modified method which proved the higher speed and efficiency of this method. All the 80 polymorphic EST-SSRs were mapped on soybean physical map through in silico mapping and 15 markers were integrated on a genetic map constructed in previous study. A software named hpSSR (high polymorphic SSR) was programmed based on the concept of the up-built method for EST-SSR development. This method is not only pragmatic for EST-SSR exploitation in soybean, but also effective for the development of the marker in other species if the redundancy EST data is available.
Bibliography:ZHAO Xue,CHANG Wei,HAN Ying-peng,TENG Wei-li and LI Wen-bin Key Laboratory of Soybean Biology,Ministry of Education/Soybean Research Institute,Northeast Agricultural University,Harbin 150030,P.R.China
10-1039/S
EST-SSR; soybean; polymorphism; genetic mapping; in silico mapping
EST-derived SSR marker has been developed in many species,but few methods of high efficiency have been reported for the exploitation of EST-SSR markers.Thus,a high efficiency method for mining millions of redundant EST data is needed.A modified method for the EST-SSR development with high efficiency was established based on the redundant EST data of soybean in this study.The method achieved its function through classifying ESTs according to the same SSR motif and detected candidate loci with redundant sequences.In this study,a total of 80 polymorphic EST-SSR markers of soybean were developed,50 of them were exploited by this modified method which proved the higher speed and efficiency of this method.All the 80 polymorphic EST-SSRs were mapped on soybean physical map through in silico mapping and 15 markers were integrated on a genetic map constructed in previous study.A software named hpSSR（high polymorphic SSR） was programmed based on the concept of the up-built method for EST-SSR development.This method is not only pragmatic for EST-SSR exploitation in soybean,but also effective for the development of the marker in other species if the redundancy EST data is available.
http://dx.doi.org/
http://www.chinaagrisci.com/Jwk_zgnykxen/fileup/PDF/2012.V11(04)-545.pdf
ISSN:2095-3119
2352-3425
DOI:10.1016/S2095-3119(12)60041-0