Sarcocystidae in wild birds of southeastern Brazil

This study aimed to identify members of the Sarcocystidae family in naturally infected wild birds at a rescue center in the state of Minas Gerais, southeastern Brazil. The heart and brain of 44 wild birds were evaluated by bioassay in mice to detect T. gondii, and extracted DNA was used for nested P...

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Published inRevista brasileira de parasitologia veterinaria Vol. 30; no. 1; p. e028520
Main Authors Rêgo, Wagner Martins Fontes do, Costa, Júlia Gatti Ladeia, Baraviera, Ramon Castro de Araujo, Pinto, Lorena Velozo, Bessa, Gabriella de Lima, Lopes, Rosálida Estevam Nazar, Silveira, Julia Angelica Gonçalves da, Vitor, Ricardo Wagner Almeida
Format Journal Article
LanguageEnglish
Published Brazil Colégio Brasileiro de Parasitologia Veterinária 01.01.2021
Colégio Brasileiro de Parasitologia Veterinaria
Subjects
Animals
Biological Assay
Bird Diseases - epidemiology
Bird Diseases - parasitology
Birds
Brazil
Coccidiosis - epidemiology
DNA, Protozoan
Mice
nested PCR
PARASITOLOGY
Polymerase Chain Reaction
RNA, Ribosomal, 18S - genetics
Sarcocystidae - genetics
Sarcocystis - genetics
Sarcocystis spp
Toxoplasma - genetics
Toxoplasma gondii
Toxoplasmosis, Animal - epidemiology
VETERINARY SCIENCES
wild birds
Brazil
wild birds
Brasil
aves silvestres
Toxoplasma gondii
nested PCR
Brazil
Sarcocystis spp
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Summary:This study aimed to identify members of the Sarcocystidae family in naturally infected wild birds at a rescue center in the state of Minas Gerais, southeastern Brazil. The heart and brain of 44 wild birds were evaluated by bioassay in mice to detect T. gondii, and extracted DNA was used for nested PCR of the 18S ribosomal DNA gene to detect members of the Sarcocystidae family. The positive samples were sequenced, assembled, edited and compared with sequences deposited in GenBank. Toxoplasma gondii was isolated from six (13.6%) out of 44 birds. Toxoplasma gondii DNA was identified in 10/44 (22.7%) of the birds. The amplified sequences exhibited 100% similarity with the DNA of the ME49 strain of T. gondii. Sarcocystis DNA (99% similarity) was identified in 5/44 (11.4%) of the birds. T. gondii and Sarcocystis spp. are common in wild birds in Minas Gerais, Brazil.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0103-846X
1984-2961
1984-2961
DOI:10.1590/S1984-296120201094