Growth rate determines activity of porphobilinogen deaminase both in nonmalignant and malignant cell lines

• Published in: Biochemical medicine and metabolic biology Vol. 40; no. 2; pp. 213 - 217
• Main Authors: Schoenfeld, Nili, Mamet, Rivka, Leibovici, Leonard, Epstein, Orit, Teitz, Yael, Atsmon, Abraham
• Format: Journal Article
• Language: English
• Published: Boston, MA Elsevier Inc 01.10.1988; San Diego, CA Academic Press; New York, NY
• Subjects: Ammonia-Lyases - metabolism; Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Cell Division; Cell Line; Enzymes and enzyme inhibitors; Fundamental and applied biological sciences. Psychology; Humans; Hydroxymethylbilane Synthase - metabolism; Lyases; Mice; Rats; Tumor Cells, Cultured - enzymology; Tumor Cells, Cultured - pathology; Cell culture; Uroporphyrinogen I synthase; Growth; Enzyme; Transformed cell; Tumor cell
• ISSN: 0885-4505; 1557-7651
• DOI: 10.1016/0885-4505(88)90121-1

PBGD activity and growth rate were determined in cultures of rat embryo fibroblasts, nontransformed and MLV MS transformed fibroblastic cell lines; NIH-3T3 cells, and in a mouse lymphosarcoma cell line [L-929]. The two parameters examined correlate positively ( P < 0.001). The results of this investigation would seem to indicate clearly that porphobilinogen deaminase activity is related to growth. However, these experiments do not rule out the possibility that malignant transformation per se also causes changes in porphobilinogen deaminase activity.