An exopolysaccharide from Trichoderma pseudokoningii and its apoptotic activity on human leukemia K562 cells

• Published in: Carbohydrate polymers Vol. 89; no. 2; pp. 701 - 708
• Main Authors: Huang, Taotao, Lin, Jun, Cao, Jianfeng, Zhang, Pengying, Bai, Yungui, Chen, Guochuang, Chen, Kaoshan
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 20.06.2012; Elsevier
• Subjects: Adjuvants; Antineoplastic agents; Antineoplastic Agents - pharmacology; Antitumor activity; Apoptosis; Apoptosis - drug effects; Applied sciences; Bax protein; Bcl-2 protein; bcl-2-Associated X Protein - genetics; Biological and medical sciences; Calcium - metabolism; Carbohydrates; Cell proliferation; Cell Proliferation - drug effects; Chemotherapy; culture media; drug therapy; Exact sciences and technology; Exopolysaccharide; exopolysaccharides; Fermentation; Fungal Polysaccharides - pharmacology; gene expression regulation; Humans; K562 Cells; Leukemia; Medical sciences; Membrane potential; Membrane Potential, Mitochondrial - drug effects; messenger RNA; Mitochondria; mitochondrial membrane; mRNA; Natural polymers; p53 protein; Pharmacology. Drug treatments; Physicochemistry of polymers; Proto-Oncogene Proteins c-bcl-2 - genetics; Reactive oxygen species; Reactive Oxygen Species - metabolism; RNA, Messenger - metabolism; Starch and polysaccharides; Trichoderma; Trichoderma pseudokoningii; Tumor Suppressor Protein p53 - genetics; Exopolysaccharide; Leukemia; Apoptosis; Antineoplastic agent; Trichoderma; Experimental study; Biological activity; Fungi; Plant origin; Oside polymer; Fungi Imperfecti; Mechanism of action; Tumor cell
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2012.03.079

► An exopolysaccharide (EPS) was isolated from culture medium of Trichoderma species. ► EPS has anticancer activity in a time- and concentration- dependent manner. ► EPS induced human leukemia K562 cell apoptosis by the mitochondrial pathways. ► EPS has a potential application as an adjuvant therapy agent against human leukemia. In this study, a novel exopolysaccharide (EPS) was isolated from the fermentation broth of Trichoderma pseudokoningii and its anticancer activities on human leukemia K562 cells were studied. EPS could significantly inhibited K562 cells proliferation in a time- and concentration-dependent manner. Meanwhile, characteristic of apoptosis, including apoptotic morphological features and the apoptosis rate were obtained. Sequentially, the dissipation of mitochondrial membrane potential, increase production of Reactive oxygen species (ROS), enhancement of the concentration of intracellular, up-regulation of Bax and p53 mRNA, down-regulation of Bcl-2 mRNA were also detected. The results indicate that the EPS could induce of K562 cells apoptosis, primarily in involved the mitochondrial pathways. The present studies suggest that EPS could be a new potential adjuvant chemotherapeutic and chemo preventive agent against human leukemia.