Determining the total energy budget of the tick Ixodes ricinus

• Published in: Experimental & applied acarology Vol. 80; no. 4; pp. 531 - 541
• Main Authors: Alasmari, Saeed, Wall, Richard
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.04.2020; Springer Nature B.V
• Subjects: acarology; Adults; Animal Ecology; Animal Genetics and Genomics; Animal Systematics/Taxonomy/Biogeography; biochemical pathways; Biomedical and Life Sciences; Carbohydrates; climate; Climate change; Energy budget; energy metabolism; Entomology; Environmental stress; epidemiological studies; Epidemiology; females; Glycogen; Glycogens; Ixodes ricinus; Life Sciences; Lipids; males; Metabolic response; Metabolism; Metabolites; nymphs; pathogens; phenology; Phospholipids; Proteins; Spectrophotometry; Ticks; triacylglycerols; Carbohydrate; Resource acquisition; Metabolism; Glycogen; Lipid; Protein
• ISSN: 0168-8162; 1572-9702; 1572-9702
• DOI: 10.1007/s10493-020-00479-1

Precise and accessible techniques for measuring metabolic responses to environmental stress are essential to allow the likely impacts of climate and climate change on tick distribution, abundance and phenology to be predicted. A more detailed understanding of the metabolic profile of ticks may also help the complex responses to pathogen infection and effects on transmission to be evaluated. Here, a series of biochemical protocols employing spectrophotometric methods are used to determine the entire energy budget of ticks. Protein, carbohydrate, total lipid, neutral lipid and glycogen were measured in individual Ixodes ricinus nymphs and adults. Two key trends were identified: in adults, protein was relatively more abundant than in nymphs, whereas in nymphs, glycogen and carbohydrate were more abundant than in adults, with glycogen alone composing 39% of the mass of metabolites in nymphs compared to 15 and 10% in females and males, respectively. The methods used were able to successfully separate neutral lipids from the polar phospholipids and the importance of distinguishing stored from structural lipid in estimates of lipid reserves is emphasised. The results demonstrate that the spectrophotometric approaches deliver relatively rapid and reliable estimates of the total energetic budget and can be used to quantify the metabolic profiles of individual ticks, demonstrating their suitability for use in ecological and epidemiological studies.