Target modification as a molecular mechanism of pyrethroid resistance in Drosophila melanogaster

• Published in: Pesticide biochemistry and physiology Vol. 44; no. 3; pp. 183 - 190
• Main Authors: Amichot, M., Castella, C., Cuany, A., Berge, J.B., Pauron, D.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.11.1992; Elsevier
• Subjects: Biological and medical sciences; Chemical control; Control; DROSOPHILA MELANOGASTER; Fundamental and applied biological sciences. Psychology; GENE; GENES; GENETICA; GENETIQUE; INSECTICIDAS; INSECTICIDE; Invertebrates; Life Sciences; Phytopathology. Animal pests. Plant and forest protection; PIRETRINAS SINTETICAS; Protozoa. Invertebrates; PYRETHRINE DE SYNTHESE; RESISTANCE AUX MALADIES; RESISTANCE AUX PRODUITS CHIMIQUES; RESISTENCIA A LA ENFERMEDAD; RESISTENCIA QUIMICA; SECUENCIA NUCLEICA; SEQUENCE NUCLEIQUE; Insecticide; Toxicity; Insecta; Biological model; Animal origin; Pyrethroids; Receiver; Ionic channel; Drosophilidae; Knockdown action; Resistance; Target; Sensitivity resistance; Gene; Substitution; Arthropoda; Voltage; Affinity; Pesticide resistance; Molecular biology; Invertebrata; Drosophila melanogaster; Diptera; RESISTANCE; CANAL SODIUM
• ISSN: 0048-3575; 1095-9939
• DOI: 10.1016/0048-3575(92)90089-I

“Knock down” resistance (kdr) to the pyrethroid deltamethrin was investigated using susceptible (Tübingen) and resistant (TübingenDDT) strains of Drosophila melanogaster. Toxicological and pharmacological data show that resistance involves a modification of the affinity of the insecticide for its receptor site on the voltage-dependent sodium channel. Genetic studies indicate that the kdr factor is linked to the second chromosome where one sodium channel gene, sch, is located. Cloning and sequencing the alleles of this gene from both strains revealed a single substitution that may be responsible for the loss of toxicity of deltamethrin in the resistant strain.