Histamine production by cultured microglial cells of the mouse

• Published in: Neuroscience letters Vol. 305; no. 3; pp. 181 - 184
• Main Authors: KATOH, Yoshiko, NIIMI, Mitsuhiro, YAMAMOTO, Yumiko, KAWAMURA, Tomohiro, MORIMOTO-ISHIZUKA, Tomoko, SAWADA, Makoto, TAKEMORI, Hiroshi, YAMATODANI, Atsushi
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier 15.06.2001
• Subjects: 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine - analogs & derivatives; 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine - pharmacology; Animals; Biological and medical sciences; Brain - cytology; Brain - metabolism; Calcium-Calmodulin-Dependent Protein Kinases - antagonists & inhibitors; Cell Line; Enzyme Inhibitors - pharmacology; Fundamental and applied biological sciences. Psychology; Histamine - biosynthesis; Histidine Decarboxylase - genetics; Histidine Decarboxylase - metabolism; Isolated neuron and nerve. Neuroglia; Lipopolysaccharides - pharmacology; Mice; Microglia - metabolism; RNA, Messenger - metabolism; Vertebrates: nervous system and sense organs; Enzyme; Neuroglia; Rodentia; Lyases; Gene expression; In vitro; Histidine decarboxylase; Microglia; Histamine; Carbon-carbon lyases; Vertebrata; Mammalia; Enzymatic activity; Mouse; Carboxy-lyases; Lipopolysaccharide
• ISSN: 0304-3940; 1872-7972
• DOI: 10.1016/s0304-3940(01)01835-3

We previously reported that cells other than mast cells or neurons could synthesize histamine in response to lipopolysaccharide (LPS) or interleukin 1beta in the rat brain. To identify the responsible cells, we examined histidine decarboxylase (HDC) activity and the expression of HDC mRNA in GMI 6-3 mouse microglial cells. Both the activity and mRNA for HDC in GMI 6-3 cells were induced by LPS treatment, and the induction was sensitive to calmodulin-dependent kinase II inhibitor, KN62. These findings indicate that microglia is a third cell type producing histamine in the brain.