Purification of a polysaccharide from Boschniakia rossica and its synergistic antitumor effect combined with 5-Fluorouracil

• Published in: Carbohydrate polymers Vol. 89; no. 1; pp. 31 - 35
• Main Authors: Wang, Zhenghui, Wu, Baojun, Zhang, Xianghong, Xu, Min, Chang, Huimin, Lu, Xiaoyun, Ren, Xiaoyong
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 05.06.2012; Elsevier
• Subjects: Adjuvant; Animals; Antineoplastic agents; Antineoplastic Agents - administration & dosage; Antineoplastic Agents - isolation & purification; Antineoplastic Agents - pharmacology; Antineoplastic Combined Chemotherapy Protocols - therapeutic use; Antitumor; Applied sciences; Biological and medical sciences; blood serum; Boschniakia rossica; carbohydrate content; Cell Line, Tumor; Cell Proliferation - drug effects; Chemotherapy; cytotoxicity; dose response; drug therapy; Exact sciences and technology; fluorouracil; Fluorouracil - administration & dosage; gel chromatography; immunity; Immunomodulatory; interferon-gamma; interleukin-2; Interleukin-2 - metabolism; intragastric administration; Killer Cells, Natural - drug effects; Male; Medical sciences; mice; Mice, Inbred ICR; molecular weight; Natural polymers; Neoplasms - drug therapy; Neoplasms - pathology; nucleic acids; Orobanchaceae; Pharmacology. Drug treatments; Physicochemistry of polymers; Phytotherapy; Polysaccharide; polysaccharides; Polysaccharides - administration & dosage; Polysaccharides - isolation & purification; Polysaccharides - pharmacology; secretion; spleen; Spleen - cytology; Spleen - drug effects; Starch and polysaccharides; synergism; T-lymphocytes; Tumor Necrosis Factor-alpha - metabolism; Adjuvant; Immunomodulatory; Boschniakia rossica; Polysaccharide; Antitumor; Antineoplastic agent; Cell proliferation; Splenocyte; Sarcoma 180; Synergism; Medicinal plant; Chinese medicine; Folk medicine; Dicotyledones; Angiospermae; Fluorouracil; Immunostimulation; Rodentia; Experimental study; Biological activity; Natural killer cell; Water soluble compound; In vivo; Vertebrata; Mammalia; Mouse; Tumor necrosis factor; Animal; Plant origin; Orobanchaceae; Oside polymer; Spermatophyta
• ISSN: 0144-8617; 1879-1344; 1879-1344
• DOI: 10.1016/j.carbpol.2012.02.024

► Boschniakia rossica is a traditional Chinese herbal medicine. ► A polysaccharide was prepared and its chemical analysis was preliminarily made. ► BRP alone or combined with 5-FU could inhibit S180 tumor growth. ► Damage to the immune system caused by 5-FU in S180-bearing mice was mitigated by BRP. ► BRP combined with 5-FU presented synergistic effects on antitumor activity in S180 tumor bearing mice. Current study we purified a polysaccharide (BRP) from Boschniakia rossica and the antitumor effects of BRP alone or combined with 5-Fluorouracil (5-FU) was examined in S180 tumor bearing mice by intragastric administration. The high performance size-exclusion chromatography (HPGEC) analysis showed that BRP was a homogeneous polysaccharide and had a molecular weight of 2.2×104Da. Total carbohydrate content in BRP was determined to be 96.9%, without the presence of protein and nucleic acid. BRP alone or combined with 5-FU could significantly inhibit Sarcoma-180 (S180) tumor growth and increase the spleen index in a dose-dependent manner. Meanwhile a synergistic effect was observed in boosting various immunity functions when the tumor bearing mice receiving BRP plus 5-FU administration, such as stimulating lymphocytes proliferation, increasing NK cell cytotoxicity, enhancing serum interleukin-2 (IL-2) and interferon-gamma (TNF-γ) secretion, as well as augmenting CD4+ and CD8+ spleen T lymphocytes subsets. The results showed that BRP combined with 5-FU presented synergistic effects on antitumor activity in tumor bearing mice. In conclusion, the combination of BRP may boost the suppressed immunity in tumor bearing mice subject to 5-FU chemotherapy, and could serve as a new, promising approach for cancer treatment.