Calculation of protein extinction coefficients from amino acid sequence data

• Published in: Analytical biochemistry Vol. 182; no. 2; pp. 319 - 326
• Main Authors: Gill, Stanley C., von Hippel, Peter H.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.11.1989; Elsevier
• Subjects: Amino Acid Sequence; Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; General aspects, investigation methods; Humans; Molecular Weight; Proteins; Proteins - analysis; Spectrophotometry, Ultraviolet - methods; Intermolecular interaction; Proteins; Spectrometry; Ligand; Method; Aminoacid sequence; Quantitative analysis; Extinction index
• ISSN: 0003-2697; 1096-0309
• DOI: 10.1016/0003-2697(89)90602-7

Quantitative study of protein-protein and protein-ligand interactions in solution requires accurate determination of protein concentration. Often, for proteins available only in “molecular biological” amounts, it is difficult or impossible to make an accurate experimental measurement of the molar extinction coefficient of the protein. Yet without a reliable value of this parameter, one cannot determine protein concentrations by the usual uv spectroscopic means. Fortunately, knowledge of amino acid residue sequence and protomer molecular weight (and thus also of amino acid composition) is generally available through the DNA sequence, which is usually accurately known for most such proteins. In this paper we present a method for calculating accurate (to ±5% in most cases) molar extinction coefficients for proteins at 280 nm, simply from knowledge of the amino acid composition. The method is calibrated against 18 “normal” globular proteins whose molar extinction coefficients are accurately known, and the assumptions underlying the method, as well as its limitations, are discussed.