Genetic diversity of chloroquine-resistant Plasmodium vivax parasites from the western Brazilian Amazon

• Published in: Memórias do Instituto Oswaldo Cruz Vol. 109; no. 7; pp. 948 - 951
• Main Authors: Lizcano, Omaira Vera, Resende, Sarah Stela, Chehuan, Yonne F, Lacerda, Marcus V G, Brito, Cristiana F A, Zalis, Mariano G
• Format: Journal Article
• Language: English
• Published: Brazil Instituto Oswaldo Cruz, Ministério da Saúde 01.11.2014; Fundação Oswaldo Cruz (FIOCRUZ)
• Subjects: Brazil - epidemiology; chloroquine; Chloroquine - pharmacology; DNA, Protozoan - isolation & purification; Drug Resistance - genetics; Endemic Diseases - statistics & numerical data; Genetic Markers; Genetic Variation; Humans; Malaria, Vivax - blood; Malaria, Vivax - epidemiology; Parasitic Sensitivity Tests; PARASITOLOGY; Phenotype; Plasmodium vivax; Plasmodium vivax - drug effects; Plasmodium vivax - genetics; Polymerase Chain Reaction; Random Allocation; resistance; Short Communication; TROPICAL MEDICINE; Brazil; chloroquine; Plasmodium vivax; resistance
• ISSN: 0074-0276; 1678-8060; 1678-8060; 0074-0276
• DOI: 10.1590/0074-0276140241

The molecular basis of Plasmodium vivax chloroquine (CQ) resistance is still unknown. Elucidating the molecular background of parasites that are sensitive or resistant to CQ will help to identify and monitor the spread of resistance. By genotyping a panel of molecular markers, we demonstrate a similar genetic variability between in vitro CQ-resistant and sensitive phenotypes of P. vivax parasites. However, our studies identified two loci (MS8 and MSP1-B10) that could be used to discriminate between both CQ-susceptible phenotypes among P. vivax isolates in vitro. These preliminary data suggest that microsatellites may be used to identify and to monitor the spread of P. vivax-resistance around the world.