Integrative growth physiology and transcriptome profiling of probiotic Limosilactobacillus reuteri KUB-AC5

• Published in: PeerJ (San Francisco, CA) Vol. 9; p. e12226
• Main Authors: Jatuponwiphat, Theeraphol, Namrak, Thanawat, Nitisinprasert, Sunee, Nakphaichit, Massalin, Vongsangnak, Wanwipa
• Format: Journal Article
• Language: English
• Published: San Diego PeerJ, Inc 05.10.2021; PeerJ Inc
• Subjects: Acclimatization; Amino acids; Bacteria; Bioinformatics; Biotechnology; Carbohydrate metabolism; Carbon; Carbon sources; Cell culture; Fermentation; Gene expression; Gene regulation; Genomes; Genomics; Glucose; Growth physiology; Inhibition effects; Levan; Levansucrase; Limosilactobacillus reuteri KUB-AC5; Metabolic response; Metabolism; Metabolites; Microbiology; Molecular Biology; Probiotics; Proteins; Salmonella; Sucrose; Transcription; Transcriptome profiling; Transcriptomes; United States > US
• ISSN: 2167-8359; 2167-8359
• DOI: 10.7717/peerj.12226

Limosilactobacillus reuteri KUB-AC5 has been widely used as probiotic in chicken for Salmonella reduction. However, a preferable carbon source and growth phase is poorly characterized underlying metabolic responses on growth and inhibition effects of L. reuteri KUB-AC5. This study therefore aimed to investigate transcriptome profiling of L. reuteri KUB-AC5 revealing global metabolic responses when alteration of carbon sources and growth phases. Interestingly, L. reuteri KUB-AC5 grown under sucrose culture showed to be the best for fast growth and inhibition effects against Salmonella Enteritidis S003 growth. Towards the transcriptome profiling and reporter proteins/metabolites analysis, the results showed that amino acid transport via ABC systems as well as sucrose metabolism and transport are key metabolic responses at Logarithmic (L)-phase of L. reuteri KUB-AC5 growth. Considering the Stationary (S)-phase, we found the potential reporter proteins/metabolites involved in carbohydrate metabolism e.g ., levansucrase and levan. Promisingly, levansucrase and levan were revealed to be candidates in relation to inhibition effects of L. reuteri KUB-AC5. Throughout this study, L. reuteri KUB-AC5 had a metabolic control in acclimatization to sucrose and energy pools through transcriptional co-regulation, which supported the cell growth and inhibition potentials. This study offers a perspective in optimizing fermentation condition through either genetic or physiological approaches for enhancing probiotic L. reuteri KUB-AC5 properties.