Phylogeny of the Falconidae (Aves): a comparison of the efficacy of morphological, mitochondrial, and nuclear data

• Published in: Molecular phylogenetics and evolution Vol. 32; no. 1; pp. 101 - 109
• Main Authors: Griffiths, Carole S., Barrowclough, George F., Groth, Jeff G., Mertz, Lisa
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.07.2004
• Subjects: Animals; Aves; Birds - genetics; Cell Nucleus - genetics; Cell Nucleus - metabolism; Codon; Cytochromes b - genetics; DNA - genetics; DNA, Mitochondrial - genetics; Exons; Falconidae; Genes, RAG-1 - genetics; Genetic Variation; Micrastur; mtDNA; Multiple data sets; Phylogenetics; Phylogeny; RAG-1; Sequence Analysis, DNA; Time Factors; Phylogenetics; Multiple data sets; Falconidae; Aves; mtDNA; RAG-1
• ISSN: 1055-7903; 1095-9513
• DOI: 10.1016/j.ympev.2003.11.019

We sequenced 2800+ bp of the RAG-1 exon for representatives of all the currently recognized genera in the avian family Falconidae. A phylogenetic analysis of these data was compared to prior analyses of mitochondrial (cytochrome- b) and morphological (syringeal) data. The nuclear RAG-1 sequences produced results that were in agreement with the morphological results, but differed from the mitochondrial results with regard to monophyly of the genus Micrastur. A reanalysis of the cytochrome- b ( cyt-b) data suggested that this result was due to heterogeneity in base composition. Comparisons of data quality and quantity across the three data sets indicate that the nuclear DNA sequences and the morphological data have similar consistency and retention indices as well as noise distributions that are superior to those of cyt-b. However, the RAG-1 data identify more nodes with high bootstrap support indices than do either morphology or mitochondrial sequences. In the final assessment, RAG-1 sequences were superior in phylogenetic utility both to syringeal morphology (because of sheer number of characters) and to cyt-b sequences (because of reduced noise and homogeneity of base composition, but in spite of having many fewer characters).