Isolation of porcine follicular fluid inhibin of 32K daltons

• Published in: Biochemical and biophysical research communications Vol. 129; no. 2; pp. 396 - 403
• Main Authors: Miyamoto, Kaoru, Hasegawa, Yoshihisa, Fukuda, Masaki, Nomura, Mitsuo, Igarashi, Masao, Kangawa, Kenji, Matsuo, Hisayuki
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 14.06.1985; Elsevier
• Subjects: Animals; Applied sciences; Biological Assay; Exact sciences and technology; Female; Inhibins - analysis; Inhibins - isolation & purification; Molecular Weight; Other techniques and industries; Ovarian Follicle - analysis; Protein Conformation; Swine; RP-HPLC; FPLC; PMSF; Mr; FSH; LH; CHAPS; SDS PAGE; TFA
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/0006-291X(85)90164-0

Purification of ovarian inhibin from porcine follicular fluid was performed by using an bioassay based upon the suppression of spontaneous FSH release from cultured cells of rat anterior pituitary. The presence in the follicular fluid of four molecular forms of inhibin activity corresponding to Mr 100K, 80K, 55K and 32K was revealed by SDS-gel electrophroresis under non-reducing conditions. The smallest inhibin amongst them, named 32K inhibin, eliciting about 70% of the total activity in the follicular fluid, was separated by gel filtration in the presence of 8 M urea. By subsequent ion-exchange chromatography, followed by RP-HPLC, 32K inhibin was purified to homogeneity with a 8,000 fold purification factor in a yield of 12%. The purified 32K inhibin was found to comprise two polypeptide subunits (Mr 20K and 13K), linked by disulfide bridges and to specifically suppress the secretion of FSH, but not of LH from the pituitary cells.