Cellulose acetate 1,2-benzenedicarboxylate inhibits infection by cell-free and cell-associated primary HIV-1 isolates

• Published in: AIDS research and human retroviruses Vol. 22; no. 5; p. 411
• Main Authors: Lu, Hong, Zhao, Qian, Wallace, Greg, Liu, Shuwen, He, Yuxian, Shattock, Robin, Neurath, A Robert, Jiang, B Shibo
• Format: Journal Article
• Language: English
• Published: United States 01.05.2006
• Subjects: Anti-HIV Agents - pharmacology; Cell Line; Cell-Free System - drug effects; Cell-Free System - metabolism; Cell-Free System - virology; Cells, Cultured; Cellulose - analogs & derivatives; Cellulose - pharmacology; Cervix Uteri - cytology; Cervix Uteri - drug effects; Cervix Uteri - metabolism; Cervix Uteri - virology; Female; HIV Infections - prevention & control; HIV Infections - virology; HIV-1 - drug effects; HIV-1 - metabolism; Humans; Leukocytes, Mononuclear - drug effects; Leukocytes, Mononuclear - metabolism; Leukocytes, Mononuclear - virology; Macrophages - drug effects; Macrophages - metabolism; Macrophages - virology
• ISSN: 0889-2229
• DOI: 10.1089/aid.2006.22.411

Cellulose acetate 1,2-benzenedicarboxylate (CAP), a pharmaceutical excipient used for enteric film coating of capsules and tablets, was previously shown to have potent inhibitory activity against infection by human immunodeficiency virus type 1 (HIV-1) T cell line-adapted (TCLA) strains. In the present study, we determined the inhibitory activity of CAP against infection by cell-free and cell-associated primary HIV-1 isolates with distinct genotypes and biotypes in cervical explants, peripheral blood mononuclear cells (PBMCs), monocytederived macrophages (MDMs), and CEMx174 5.25M7 cells. CAP blocked infection by cell-free and cell-associated HIV-1 in cervical explants. It inhibited infection by cell-free primary HIV-1 isolates (clades A to G and group O) in PBMCs, MDMs, and CEMx174 5.25M7 cells and blocked transmissions of the cell-associated primary HIV-1 isolates from dendritic cells (DCs) to PBMCs, from MDMs to PBMCs, and from PBMCs to CEMx174 5.25M7 cells. The inhibitory activity of CAP on infection by the cell-free and cell-associated primary HIV-1 isolates is independent of viral subtypes and coreceptor usage. These data suggest that CAP is a good microbicide candidate that can be further developed for preventing sexual transmission of HIV-1.