pT181 plasmid replication is regulated by a countertranscript-driven transcriptional attenuator

• Published in: Cell Vol. 59; no. 2; pp. 395 - 404
• Main Authors: Novick, Richard P., Iordanescu, Serban, Projan, Steven J., Kornblum, John, Edelman, Irit
• Format: Journal Article
• Language: English
• Published: Cambridge, MA Elsevier Inc 20.10.1989; Cell Press
• Subjects: Bacterial Proteins - genetics; Base Sequence; Biological and medical sciences; DNA Replication; DNA-Binding Proteins - genetics; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Bacterial; Genes, Bacterial; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Nucleic Acid Conformation; Plasmids; Restriction Mapping; RNA - genetics; RNA, Antisense; RNA, Messenger - antagonists & inhibitors; RNA, Messenger - genetics; Staphylococcus aureus - genetics; Transcription, Genetic; Transcription. Transcription factor. Splicing. Rna processing; Plasmid; Transcription termination; Construction; Messenger RNA; Transcription attenuation; Bacteria; Micrococcales; Antisense RNA; Replication; Micrococcaceae; Staphylococcus
• ISSN: 0092-8674; 1097-4172
• DOI: 10.1016/0092-8674(89)90300-0

pT181 is the prototype of a family of staphylococcal plasmids that regulate their replication by means of antisense RNAs (countertranscripts) that block expression of the plasmid-coded initator protein. In this paper, we show that the pT181 countertranscripts induce premature termination (attenuation) of the initiator mRNA by promoting the formation of a termination-causing hairpin just 5′ to the initiator start codon. In the absence of the countertranscripts, an upstream sequence, the preemptor, pairs with the proximal arm of the terminator hairpin, preventing termination and permitting transcription of the initiator gene. This system thus differs from the classical attenuators in that attenuation is driven by antisense RNAs rather than by tRNA-induced stalling of ribosomes.