Elucidation of glycolipid structure by proton nuclear magnetic resonance spectroscopy

• Published in: Chemistry and physics of lipids Vol. 42; no. 1; pp. 27 - 48
• Main Authors: Yu, Robert K., Koerner, Theodore A.W., Neel Scarsdale, J., Prestegard, James H.
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 15.12.1986; Elsevier Science
• Subjects: Analytical, structural and metabolic biochemistry; Biological and medical sciences; Carbohydrate Conformation; Carbohydrate Sequence; Fundamental and applied biological sciences. Psychology; Gangliosides; Glycolipids; glycosphingolipids; Lipids; Magnetic Resonance Spectroscopy; Oligosaccharides; Other biological molecules; proton NMR; secondary structure; solution conformation; secondary structure; gangliosides; solution conformation; proton NMR; glycosphingolipids; Sphingoglycolipid; NMR spectrometry; Ganglioside; Conformation
• ISSN: 0009-3084; 1873-2941
• DOI: 10.1016/0009-3084(86)90041-1

The primary structure of the oligosaccharide moiety of a glycosphingolipid can be elucidated by employing high-field proton nuclear magnetic resonance (NMR) spectroscopy. Information with respect to the composition and configuration of its sugar residues, and the sequence and linkage sites of the oligosaccharide chain can be obtained by employing a variety of one- and two-dimensional techniques. The latter include both scalar and dipolar correlated two-dimensional NMR spectroscopy. These techniques are also useful in establishing the solution conformation (secondary structure) of the oligosaccharide moiety. Examples in utilizing these techniques in elucidating the primary and secondary structures of glycolipids are presented.